A Guide to Analysis of Relative Synaptic Protein Abundance by Quantitative Fluorescent Western Blotting
| العنوان: | A Guide to Analysis of Relative Synaptic Protein Abundance by Quantitative Fluorescent Western Blotting |
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| المؤلفون: | Heidi K. Müller |
| المصدر: | Müller, H K 2022, A Guide to Analysis of Relative Synaptic Protein Abundance by Quantitative Fluorescent Western Blotting . in Methods in Molecular Biology . Humana Press, New York, Methods in Molecular Biology, vol. 2417, pp. 89-98 . https://doi.org/10.1007/978-1-0716-1916-2_7 Methods in Molecular Biology ISBN: 9781071619155 |
| بيانات النشر: | Humana Press, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Normalization, Phosphoprotein detection, Best practice, Fluorescent Western Blotting, Nitrocellulose membrane, Multiplexing, Quantitative, Synaptic proteins |
| الوصف: | The introduction of fluorescent detection systems has revolutionized the applicability of Western blotting for quantitative protein expression analyses. The fundamental premise behind fluorescent Western blotting is the combination of distinct fluorescent dye-conjugated secondary antibodies and high performance digital imaging solutions in which the fluorescence signal is directly proportional to the amount of protein enabling quantitative measurements and simultaneous detection of several target proteins. This aspect of Western blotting is now widely used, especially in preclinical research, to detect quantitative changes in protein levels and phosphorylation status between experimental groups. This chapter provides a detailed step-by-step guide for best practice procedures during the entire process from sample preparation, SDS polyacrylamide gel electrophoresis to electrotransfer of proteins and highlights approaches that can be applied to increase data output. |
| اللغة: | English |
| ردمك: | 978-1-07-161915-5 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9dad89bcc413a5d469acfc6b38c55d05 https://pure.au.dk/portal/da/publications/a-guide-to-analysis-of-relative-synaptic-protein-abundance-by-quantitative-fluorescent-western-blotting(9cd073f5-5bc3-48a7-9b05-cfb43a9522fe).html |
| حقوق: | RESTRICTED |
| رقم الأكسشن: | edsair.doi.dedup.....9dad89bcc413a5d469acfc6b38c55d05 |
| قاعدة البيانات: | OpenAIRE |
| ردمك: | 9781071619155 |
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