High-Throughput Fluorescent Tagging of Full-Length Arabidopsis Gene Products in Planta
| العنوان: | High-Throughput Fluorescent Tagging of Full-Length Arabidopsis Gene Products in Planta |
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| المؤلفون: | S. Narasimha Chary, Amitabh Mohanty, David A. Jackson, Jianxiong Li, Shijun Li, Charles D. Kopec, Natasha V. Raikhel, Guo-Wei Tian, Seung Y. Rhee, Brigitte Paap, David W. Ehrhardt, Georgia Drakakaki, Vitaly Citovsky |
| المصدر: | Plant Physiology. 135:25-38 |
| بيانات النشر: | Oxford University Press (OUP), 2004. |
| سنة النشر: | 2004 |
| مصطلحات موضوعية: | Nuclear Envelope, Physiology, Molecular Sequence Data, Arabidopsis, Plant Science, Computational biology, Fluorescence, Cytosol, Cell Wall, Gene expression, Peroxisomes, Genetics, Plastids, Gene, Cytoskeleton, biology, Arabidopsis Proteins, Gene Expression Profiling, Cell Membrane, Plasmodesmata, Promoter, Breakthrough Technologies, Plants, Genetically Modified, biology.organism_classification, Subcellular localization, Protein subcellular localization prediction, Luminescent Proteins, Proteome, Function (biology) |
| الوصف: | We developed a high-throughput methodology, termed fluorescent tagging of full-length proteins (FTFLP), to analyze expression patterns and subcellular localization of Arabidopsis gene products in planta. Determination of these parameters is a logical first step in functional characterization of the approximately one-third of all known Arabidopsis genes that encode novel proteins of unknown function. Our FTFLP-based approach offers two significant advantages: first, it produces internally-tagged full-length proteins that are likely to exhibit native intracellular localization, and second, it yields information about the tissue specificity of gene expression by the use of native promoters. To demonstrate how FTFLP may be used for characterization of the Arabidopsis proteome, we tagged a series of known proteins with diverse subcellular targeting patterns as well as several proteins with unknown function and unassigned subcellular localization. |
| تدمد: | 1532-2548 0032-0889 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9de80e8048af53681bc0274811d17c7d https://doi.org/10.1104/pp.104.040139 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....9de80e8048af53681bc0274811d17c7d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15322548 00320889 |
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