Transcriptional profiling of breast cancer cells in response to mevinolin: Evidence of cell cycle arrest, DNA degradation and apoptosis
العنوان: | Transcriptional profiling of breast cancer cells in response to mevinolin: Evidence of cell cycle arrest, DNA degradation and apoptosis |
---|---|
المؤلفون: | Ali M. Mahmoud, Yazeed A. Al-Sheikh, Hany A. El-Shemy, Junkyu Han, Mourad A. M. Aboul-Soud, Ahmed M. Al-Abd |
المصدر: | International Journal of Oncology |
بيانات النشر: | Spandidos Publications, 2016. |
سنة النشر: | 2016 |
مصطلحات موضوعية: | p53, 0301 basic medicine, Cancer Research, Programmed cell death, Cell cycle checkpoint, natural products, DNA repair, Cell, Antineoplastic Agents, Apoptosis, Breast Neoplasms, DNA Fragmentation, Biology, Bioinformatics, 03 medical and health sciences, 0302 clinical medicine, medicine, Humans, Lovastatin, Cell Proliferation, Oligonucleotide Array Sequence Analysis, Sequence Analysis, RNA, Cell growth, Gene Expression Profiling, mevinolin, Articles, Cell Cycle Checkpoints, Cell cycle, Gene Expression Regulation, Neoplastic, 030104 developmental biology, medicine.anatomical_structure, Oncology, Cell culture, 030220 oncology & carcinogenesis, MCF-7 Cells, Cancer research, Female, MCF-7, microarray |
الوصف: | The merging of high-throughput gene expression techniques, such as microarray, in the screening of natural products as anticancer agents, is considered the optimal solution for gaining a better understanding of the intervention mechanism. Red yeast rice (RYR), a Chinese dietary product, contains a mixture of hypocholesterolemia agents such as statins. Typically, statins have this effect via the inhibition of HMG‑CoA reductase, the key enzyme in the biosynthesis of cholesterol. Recently, statins have been shown to exhibit various beneficial antineoplastic properties through the disruption of tumor angiogenesis and metastatic processes. Mevinolin (MVN) is a member of statins and is abundantly present in RYR. Early experimental trials suggested that the mixed apoptotic/necrotic cell death pathway is activated in response to MVN exposure. In the current study, the cytotoxic profile of MVN was evaluated against MCF‑7, a breast cancer‑derived cell line. The obtained results indicated that MVN‑induced cytotoxicity is multi‑factorial involving several regulatory pathways in the cytotoxic effects of MVN on breast cancer cell lines. In addition, MVN‑induced transcript abundance profiles inferred from microarrays showed significant changes in some key cell processes. The changes were predicted to induce cell cycle arrest and reactive oxygen species generation but inhibit DNA repair and cell proliferation. This MVN‑mediated multi‑factorial stress triggered specific programmed cell death (apoptosis) and DNA degradation responses in breast cancer cells. Taken together, the observed MVN‑induced effects underscore the potential of this ubiquitous natural compound as a selective anticancer activity, with broad safety margins and low cost compared to benchmarked traditional synthetic chemotherapeutic agents. Additionally, the data support further pre‑clinical and clinical evaluations of MVN as a novel strategy to combat breast cancer and overcome drug resistance. |
تدمد: | 1791-2423 1019-6439 |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9e459bdf191764f9513038a5fb7d2735 https://doi.org/10.3892/ijo.2016.3418 |
حقوق: | OPEN |
رقم الأكسشن: | edsair.doi.dedup.....9e459bdf191764f9513038a5fb7d2735 |
قاعدة البيانات: | OpenAIRE |
تدمد: | 17912423 10196439 |
---|