Signalling via CD28 of human naive neonatal T lymphocytes
| العنوان: | Signalling via CD28 of human naive neonatal T lymphocytes |
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| المؤلفون: | Luke A. J. O'Neill, D. J. Reen, Jaythoon Hassan, U. Pattison, Sarah O'Neill |
| المصدر: | Clinical and Experimental Immunology. 102:192-198 |
| بيانات النشر: | Oxford University Press (OUP), 1995. |
| سنة النشر: | 1995 |
| مصطلحات موضوعية: | Adult, Cellular immunity, T-Lymphocytes, T cell, Immunology, CD2 Antigens, Biology, Lymphocyte Activation, CD28 Antigens, Antigen, medicine, Humans, Immunology and Allergy, RNA, Messenger, Cell growth, Infant, Newborn, NF-kappa B, Antibodies, Monoclonal, CD28, T lymphocyte, Fetal Blood, NFKB1, medicine.anatomical_structure, biology.protein, Interleukin-2, Antibody, Research Article |
| الوصف: | SUMMARYAccessory molecules play a crucial role in the development of the T cell response to antigenic challenge. We have examined the role of CD28 in modulating the‘naive’ neonatal T cell response to anti-CD2-mediated activation. To compare the role of CD28, neonatal and adult T cells were stimulated with a pair of mitogenic anti-CD2 antibodies in the presence or absence or anti-CD28 MoAb. With anti-CD2 alone, neonatal T cells proliferated slightly but produced no detectable IL-2, whereas adult T cells proliferated vigorously, with significant IL-2 production. Costimulation with anti-CD28 MoAb greatly enhanced the proliferative response of neonatal T cells to levels equivalent to those of adult T cells, whereas adult T cells showed only slight increases. Although IL-2 secretion was increased in the presence of anti-CD28 MoAb. neonatal T cell IL-2 production remained lower than in adults. In contrast, enhancement of IL-2 mRNA expression in neonates was similar to adult levels. Anti-CD28 MoAb costimulation increased NFkB levels in neonates, albeit to levels lower than that of adults. The cellular mechanism governing the diminished proliferative response of neonatal T lymphocytes to anti-CD2 may therefore be due to decreased NFkB induction, reduced IL-2 mRNA expression and deficient IL-2 production. Although anti-CD28 MoAb costimulation enhances all of the above signals, NFkB and IL-2 levels remain lower than in adults, suggesting the need for further activation requirements in the neonate. |
| تدمد: | 1365-2249 0009-9104 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9eb085961f3c1394fd63617415d0ebbc https://doi.org/10.1111/j.1365-2249.1995.tb06655.x |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....9eb085961f3c1394fd63617415d0ebbc |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13652249 00099104 |
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