Glycolytic reprograming in Salmonella counters NOX2-mediated dissipation of ΔpH
| العنوان: | Glycolytic reprograming in Salmonella counters NOX2-mediated dissipation of ΔpH |
|---|---|
| المؤلفون: | Sangeeta Chakraborty, Ju-Sim Kim, Prerak T. Desai, Steffen Porwollik, Michael McClelland, Liam F. Fitzsimmons, Lin Liu, Andrés Vázquez-Torres |
| المصدر: | Nature communications, vol 11, iss 1 Nature Communications, Vol 11, Iss 1, Pp 1-11 (2020) Nature Communications |
| بيانات النشر: | eScholarship, University of California, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Salmonella typhimurium, 0301 basic medicine, Bioenergetics, Cellular respiration, Science, 030106 microbiology, Respiratory chain, General Physics and Astronomy, Bacterial physiology, medicine.disease_cause, Article, General Biochemistry, Genetics and Molecular Biology, Vaccine Related, 03 medical and health sciences, Bacterial Proteins, Biodefense, medicine, Cellular microbiology, lcsh:Science, Multidisciplinary, NADPH oxidase, biology, Chemistry, Prevention, NADPH Oxidases, Bacteriology, General Chemistry, Cell biology, Respiratory burst, Oxidative Stress, Metabolic pathway, Emerging Infectious Diseases, Infectious Diseases, 030104 developmental biology, Fermentation, biology.protein, lcsh:Q, Reactive Oxygen Species, Glycolysis, Oxidation-Reduction, Intracellular, Oxidative stress |
| الوصف: | The microbial adaptations to the respiratory burst remain poorly understood, and establishing how the NADPH oxidase (NOX2) kills microbes has proven elusive. Here we demonstrate that NOX2 collapses the ΔpH of intracellular Salmonella Typhimurium. The depolarization experienced by Salmonella undergoing oxidative stress impairs folding of periplasmic proteins. Depolarization in respiring Salmonella mediates intense bactericidal activity of reactive oxygen species (ROS). Salmonella adapts to the challenges oxidative stress imposes on membrane bioenergetics by shifting redox balance to glycolysis and fermentation, thereby diminishing electron flow through the membrane, meeting energetic requirements and anaplerotically generating tricarboxylic acid intermediates. By diverting electrons away from the respiratory chain, glycolysis also enables thiol/disulfide exchange-mediated folding of bacterial cell envelope proteins during periods of oxidative stress. Thus, primordial metabolic pathways, already present in bacteria before aerobic respiration evolved, offer a solution to the stress ROS exert on molecular targets at the bacterial cell envelope. Chakraborty et al. show that phagocyte NADPH oxidase (NOX2) collapses the ΔpH of intracellular Salmonella Typhimurium, leading to oxidative damage of cell envelope proteins. Salmonella responds by shifting redox balance from respiration to glycolysis and fermentation, thereby facilitating folding of periplasmic functions. |
| وصف الملف: | application/pdf |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9ebd8d090227fbaefee826c03319f6ee https://escholarship.org/uc/item/1vz1b10t |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....9ebd8d090227fbaefee826c03319f6ee |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |