rTMS Ameliorates PTSD Symptoms in Rats by Enhancing Glutamate Transmission and Synaptic Plasticity in the ACC via the PTEN/Akt Signalling Pathway
| العنوان: | rTMS Ameliorates PTSD Symptoms in Rats by Enhancing Glutamate Transmission and Synaptic Plasticity in the ACC via the PTEN/Akt Signalling Pathway |
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| المؤلفون: | Dayun Feng, Shengxi Wu, Zhengwu Peng, Min Cai, Gao-hua Liu, Jian Wang, Rui-Guo Zhang, Jing Yang, Huaning Wang, Qingrong Tan, Haifeng Zhang |
| المصدر: | Molecular Neurobiology. |
| بيانات النشر: | Springer Science and Business Media LLC, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Receptor expression, Neuroscience (miscellaneous), Down-Regulation, Glutamic Acid, AMPA receptor, Gyrus Cinguli, Synaptic Transmission, behavioral disciplines and activities, Rats, Sprague-Dawley, Stress Disorders, Post-Traumatic, 03 medical and health sciences, Cellular and Molecular Neuroscience, 0302 clinical medicine, mental disorders, Animals, Medicine, PTEN, Tensin, Protein kinase B, Neurons, Neuronal Plasticity, Behavior, Animal, biology, business.industry, PTEN Phosphohydrolase, Glutamate receptor, Transcranial Magnetic Stimulation, 030104 developmental biology, Receptors, Glutamate, nervous system, Neurology, Synaptic plasticity, biology.protein, NMDA receptor, business, Proto-Oncogene Proteins c-akt, Neuroscience, 030217 neurology & neurosurgery, Signal Transduction |
| الوصف: | Repetitive transcranial magnetic stimulation (rTMS) is a novel physiological therapy that has been adopted to clinically treat psychiatric disorders. Our previous study indicated the potential therapeutic effect of rTMS on posttraumatic stress disorder (PTSD). However, the exact molecular mechanism is elusive. Currently, using the single prolonged stress (SPS) rat model for PTSD, we investigated the glutamatergic transmission and neural plasticity changes in the anterior cingulate cortex (ACC) after SPS induction and explored the protective effects and mechanism of rTMS treatment. We found that high-frequency rTMS (HrTMS, 15 Hz) treatment significantly relieved the impaired glutamatergic receptors in the ACC after SPS treatment by significantly increasing NMDAR and AMPAR expression. Simultaneously, HrTMS blocked inhibited neuronal phosphatase and tensin homologue on chromosome 10 (PTEN)/Akt signalling in the ACC after SPS treatment by decreasing PTEN expression and increasing Akt phosphorylation, which is critically involved in the regulation of memory and synaptic plasticity. The PTEN inhibitors bpV and small interfering RNA and the Akt inhibitor wortmannin were stereotaxically administered to the ACC after SPS treatment to advance the mechanistic study. Analysis by Western blot, double immunofluorescence, Golgi staining and behavioural tests demonstrated that the effects of rTMS on PTEN/Akt activation, glutamatergic receptor expression, neuronal synaptic plasticity and PTSD-related behaviours induced by SPS treatment were enhanced by PTEN inhibition and blocked by Akt inhibition in the ACC. Our study provides convincing evidence for the effectiveness of rTMS treatment on PTSD and suggests that its potential mechanism involves remodelling neuronal synaptic plasticity via the PTEN/Akt signalling pathway. |
| تدمد: | 1559-1182 0893-7648 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9ef3af9407f982b0d8bbe1a9d30caed9 https://doi.org/10.1007/s12035-017-0602-7 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....9ef3af9407f982b0d8bbe1a9d30caed9 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 15591182 08937648 |
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