Effects of phycoerythrin from Gracilaria lemaneiformis in proliferation and apoptosis of SW480 cells
| العنوان: | Effects of phycoerythrin from Gracilaria lemaneiformis in proliferation and apoptosis of SW480 cells |
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| المؤلفون: | Qingli Chang, Jun Ying, Huiguang Yi, Wen Zhu, Xiaofeng Zeng, Chunxia Yan, Enyong Zhang, Qiyu Bao, Teng Xu, Peizhen Li, Ruowang Pan, Sheng-Bin Li, Guangjian Yang |
| المصدر: | Oncology Reports. 36:3536-3544 |
| بيانات النشر: | Spandidos Publications, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Cancer Research, Gene Expression, Apoptosis, Flow cytometry, 03 medical and health sciences, 0302 clinical medicine, Ezrin, Cell Line, Tumor, medicine, Gracilaria, Humans, Endoplasmic Reticulum Chaperone BiP, Cell Proliferation, medicine.diagnostic_test, biology, Cell growth, Phycoerythrin, General Medicine, Cell cycle, Antineoplastic Agents, Phytogenic, Molecular biology, Cell biology, Blot, 030104 developmental biology, Oncology, Cell culture, 030220 oncology & carcinogenesis, biology.protein, Drug Screening Assays, Antitumor, Nucleophosmin |
| الوصف: | We studied phycoerythrin (PE) in human SW480 tumor cells and the underlying molecular mechanisms of action. PE inhibited cell proliferation as evidenced by CCK-8 assay. The IC50 values of phycoerythrin were 48.2 and 27.4 µg/ml for 24 and 48 h of exposure, respectively. PE induced apoptosis and cell cycle arrest in SW480 cells as observed under electron microscopy and with flow cytometry. Apoptosis increased from 5.1 (controls) to 39.0% in 80.0 µg/ml PE-treated cells. Differences in protein expression were identified using proteomic techniques. Protein spots (1018±60 and 1010±60) were resolved in PE-treated and untreated group. Forty differential protein spots were analyzed with MALDI-TOF-MS, including GRP78 and NPM1. The expression as measured by qPCR and western blotting agreed with data from two-dimensional electrophoresis. GRP78, NPM1, MTHSP75, Ezrin and Annexin A2 were decreased and HSP60 was increased after PE treatment, indicating that PE may target multiple proteins to induce apoptosis. |
| تدمد: | 1791-2431 1021-335X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9efa3e5670a2af8ea90e636501214d22 https://doi.org/10.3892/or.2016.5162 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....9efa3e5670a2af8ea90e636501214d22 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17912431 1021335X |
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