Influence of pig farming on the human's nasal microbiota: The key role of the airborne microbial communities
| العنوان: | Influence of pig farming on the human's nasal microbiota: The key role of the airborne microbial communities |
|---|---|
| المؤلفون: | Markus Hilty, Suzanne Aebi, Julia G. Kraemer, Anne Oppliger, Alban Ramette |
| المصدر: | Kraemer, Julia G; Ramette, Alban; Aebi, Suzanne; Oppliger, Anne; Hilty, Markus (2018). Influence of pig farming on the human's nasal microbiota: The key role of the airborne microbial communities. Applied and environmental microbiology, 84(6) American Society for Microbiology 10.1128/AEM.02470-17 <http://dx.doi.org/10.1128/AEM.02470-17> Applied and environmental microbiology, vol. 84, no. 6, pp. e02470-17[1-13] Applied and Environmental Microbiology |
| بيانات النشر: | American Society for Microbiology, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Swine, animal diseases, Air Microbiology, Biodiversity, microbial ecology, Applied Microbiology and Biotechnology, RNA, Ribosomal, 16S, Environmental Microbiology, Pig farming, Spotlight, 2. Zero hunger, Farmers, Ecology, Transmission (medicine), Microbiota, Human microbiome, pigs, Agriculture, Dust, Animals, Bacteria/classification, Bacteria/genetics, Bacteria/isolation & purification, Cattle, Cross-Sectional Studies, Farms, Fungi/classification, Fungi/genetics, Fungi/isolation & purification, Humans, Microbiota/genetics, Nose/microbiology, RNA, Ribosomal, 16S/genetics, medicine.anatomical_structure, Nasal Swab, Biotechnology, 030106 microbiology, microbial communities, mothur, Zoology, 610 Medicine & health, Nose, Biology, 03 medical and health sciences, Human nose, medicine, Bacteria, business.industry, Fungi, 030104 developmental biology, occupational health, 570 Life sciences, biology, business, Food Science |
| الوصف: | It has been hypothesized that the environment can influence the composition of the nasal microbiota. However, the direct influence of pig farming on the anterior and posterior nasal microbiota is unknown. Using a cross-sectional design, pig farms ( n = 28) were visited in 2014 to 2015, and nasal swabs from 43 pig farmers and 56 pigs, as well as 27 air samples taken in the vicinity of the pig enclosures, were collected. As controls, nasal swabs from 17 cow farmers and 26 non-animal-exposed individuals were also included. Analyses of the microbiota were performed based on 16S rRNA amplicon sequencing and the DADA2 pipeline to define sequence variants (SVs). We found that pig farming is strongly associated with specific microbial signatures (including alpha- and beta-diversity), which are reflected in the microbiota of the human nose. Furthermore, the microbial communities were more similar within the same farm compared to between the different farms, indicating a specific microbiota pattern for each pig farm. In total, there were 82 SVs that occurred significantly more abundantly in samples from pig farms than from cow farmers and nonexposed individuals (i.e., the core pig farm microbiota). Of these, nine SVs were significantly associated with the posterior part of the human nose. The results strongly indicate that pig farming is associated with a distinct human nose microbiota. Finally, the community structures derived by the DADA2 pipeline showed an excellent agreement with the outputs of the mothur pipeline which was revealed by procrustes analyses. IMPORTANCE The knowledge about the influence of animal keeping on the human microbiome is important. Previous research has shown that pets significantly affect the microbial communities of humans. However, the effect of animal farming on the human microbiota is less clear, although it is known that the air at farms and, in particular, at pig farms is charged with large amounts of dust, bacteria, and fungi. In this study, we simultaneously investigated the nasal microbiota of pigs, humans, and the environment at pig farms. We reveal an enormous impact of pig farming on the human nasal microbiota which is far more pronounced compared to cow farming. In addition, we analyzed the airborne microbiota and found significant associations suggesting an animal-human transmission of the microbiota within pig farms. We also reveal that microbial patterns are farm specific, suggesting that the environment influences animals and humans in a similar manner. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a044ccd5dafab2c3541bb6c4046affdf |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....a044ccd5dafab2c3541bb6c4046affdf |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |