GM-CSF-transduced B16 melanoma cells are highly susceptible to lysis by normal murine macrophages and poorly tumorigenic in immune-compromised mice
| العنوان: | GM-CSF-transduced B16 melanoma cells are highly susceptible to lysis by normal murine macrophages and poorly tumorigenic in immune-compromised mice |
|---|---|
| المؤلفون: | Zhongyun Dong, Isaiah J. Fidler, Junya Yoneda, Rakesh Kumar |
| المصدر: | Journal of Leukocyte Biology. 65:102-108 |
| بيانات النشر: | Oxford University Press (OUP), 1999. |
| سنة النشر: | 1999 |
| مصطلحات موضوعية: | Cytotoxicity, Immunologic, Male, Immunology, Melanoma, Experimental, Mice, Nude, Biology, Matrix metalloproteinase, Immunocompromised Host, Mice, Transduction, Genetic, In vivo, Tumor Cells, Cultured, medicine, Bystander effect, Animals, Immunology and Allergy, Cytotoxicity, neoplasms, Mice, Inbred BALB C, Macrophages, Melanoma, Granulocyte-Macrophage Colony-Stimulating Factor, Cell Biology, medicine.disease, In vitro, Mice, Inbred C57BL, Granulocyte macrophage colony-stimulating factor, Cancer research, Female, Tumor necrosis factor alpha, medicine.drug |
| الوصف: | Granulocyte-macrophage colony-stimulating factor (GM-CSF)-transduced B16-F10 murine melanoma cells had lower tumorigenicity in both syngeneic and nude mice than parental or LacZ-transduced (control) cells. The subcutaneous (s.c.) tumors producing GM-CSF were densely infiltrated with macrophages, whereas the control tumors were not. In vitro studies showed that GM-CSF-transduced B16 cells were susceptible to lysis mediated by nonactivated murine macrophages, whereas control B16 cells were not. Macrophage-mediated cytotoxicity against GM-CSF-transduced B16 cells was independent of the presence of NO, H2O2, O2-, tumor necrosis factor α, and matrix metalloproteinase. Coculture experiments using GM-CSF-producing and -nonproducing B16 cells demonstrated that GM-CSF produced by the transduced B16 cells activated macrophages to kill the bystander non-GM-CSF-producing tumor cells. The results suggest that GM-CSF released by tumor cells can induce macrophage-mediated cytotoxicity, which in turn can inhibit the in vivo growth of GM-CSF-transduced tumor cells. J. Leukoc. Biol. 65: 102–108; 1999. |
| تدمد: | 1938-3673 0741-5400 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a10cdfae03b47ac5e76abb4c50ee8a41 https://doi.org/10.1002/jlb.65.1.102 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....a10cdfae03b47ac5e76abb4c50ee8a41 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19383673 07415400 |
|---|