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Dependence of transfection efficiency of calcium treated Escherichia coli cells on bacterial genotype and form of Lambda DNA

التفاصيل البيبلوغرافية
العنوان: Dependence of transfection efficiency of calcium treated Escherichia coli cells on bacterial genotype and form of Lambda DNA
المؤلفون: Legina Ok, Konevega Lv, Drabkina Le, M. I. Mosevitsky
المصدر: Moleculargeneral genetics : MGG. 144(1)
سنة النشر: 1976
مصطلحات موضوعية: animal structures, Genotype, viruses, chemistry.chemical_element, Calcium, medicine.disease_cause, Coliphages, chemistry.chemical_compound, Transformation, Genetic, Species Specificity, Lysogenic cycle, Genetics, medicine, Centrifugation, Density Gradient, Escherichia coli, Molecular Biology, Lysogeny, Infectivity, Nuclease, biology, fungi, Transfection, Molecular biology, chemistry, embryonic structures, DNA, Viral, biology.protein, DNA, Circular, DNA
الوصف: The transfecting activity of linear lambda DNA is 100 times higher in calcium treated E. coli K12 (lambda i434) than in non-lysogenic strains: the levels of transfection are 1-2.10(7) and 1-2.10(5) infective centers per 1 mug of lambda DNA respectively. The high efficiency of lysogenic cells transfection is not due to the spontaneously liberated "helper" phage. Evidently, it is called forth by transfecting DNA-prophage recombination or/and by inhibition of nuclease activity in lysogenic cells. Both ring forms lambda DNA (supercoiled and open circles) show very low infectivity, if any, in calcinated cells.
تدمد: 0026-8925
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a1290d837eabc39eac403e419a90a921
https://pubmed.ncbi.nlm.nih.gov/772416
حقوق: CLOSED
رقم الأكسشن: edsair.doi.dedup.....a1290d837eabc39eac403e419a90a921
قاعدة البيانات: OpenAIRE
الوصف
تدمد:00268925