Identification of proteins associated with two diverse Caulobacter phicbkvirus particles
| العنوان: | Identification of proteins associated with two diverse Caulobacter phicbkvirus particles |
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| المؤلفون: | Fanchao Zhu, Kiesha Wilson, Bert Ely, Sixue Chen, Ran Zheng |
| المصدر: | Arch Virol |
| بيانات النشر: | Springer Science and Business Media LLC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Genetics, 0303 health sciences, Lysis, biology, Caulobacter, 030306 microbiology, Caulobacter crescentus, Genomics, Genome, Viral, General Medicine, Siphoviridae, biology.organism_classification, Genome, Virology, Article, Bacteriophage, Viral Proteins, 03 medical and health sciences, Bacteriophages, Gene, Function (biology), 030304 developmental biology |
| الوصف: | Genomic evolution among bacteriophages infecting Caulobacter crescentus is inevitable. However, the conservation of the proteins associated with intact phage particles has not been investigated. In this study, we compared the structural proteins associated with two genomically diverse but morphologically similar C. crescentus-infecting bacteriophages, phiCbK and CcrSC. We were able to detect more than 20 proteins that are part of the bacteriophage particle in both phages, and we were able to identify a small number of proteins that were found in only one of the two phage particles. All but one of the genes coding for these structural proteins were located in a region of the genome that had been designated a structural region, confirming the idea that the genes in these phage genomes are clustered according to their function. During the purification process, we also discovered that phiCbk has a replication complex that can be recovered from the cell lysate, and this complex allowed us to identify many of the phage proteins involved in phage genome replication. |
| تدمد: | 1432-8798 0304-8608 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a188c583ad9eef73057ca649eaab679e https://doi.org/10.1007/s00705-020-04707-2 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....a188c583ad9eef73057ca649eaab679e |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14328798 03048608 |
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