Genomic Identification and Functional Analysis of JHAMTs in the Pond Wolf Spider, Pardosa pseudoannulata
| العنوان: | Genomic Identification and Functional Analysis of JHAMTs in the Pond Wolf Spider, Pardosa pseudoannulata |
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| المؤلفون: | Fang-Fang Li, Zewen Liu, Zhi-Ming Yang, Yong Wu, Na Yu, Zhang-Jin Zhou |
| المصدر: | International Journal of Molecular Sciences, Vol 22, Iss 11721, p 11721 (2021) International Journal of Molecular Sciences Volume 22 Issue 21 |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Male, QH301-705.5, media_common.quotation_subject, Wolf spider, Gene Expression, Pardosa pseudoannulata, Zoology, Insect, Biology, phylogeny, Article, Catalysis, Arthropod Proteins, Inorganic Chemistry, RNA interference, Phylogenetics, Animals, Physical and Theoretical Chemistry, Biology (General), Molecular Biology, QD1-999, Spectroscopy, Chromosome 13, media_common, Spider, Phylogenetic tree, JHAMT, Organic Chemistry, Spiders, Methyltransferases, General Medicine, biology.organism_classification, Computer Science Applications, Juvenile Hormones, Chemistry, gene expansion, Juvenile hormone, Female, Moulting |
| الوصف: | Juvenile hormone (JH) plays a critical role in many physiological activities of Arthropoda. Juvenile hormone acid methyltransferase (JHAMT) is involved in the last steps of JH biosynthesis as an important rate-limiting enzyme. In recent studies, an increasing number of JHAMTs were identified in arthropods, but no JHAMT was reported in spiders. Herein, eight JHAMTs were identified in the pond wolf spider, Pardosa pseudoannulata, all containing the well conserved S-adenosyl-L-methionine binding motif. JHAMT-1 and the other seven JHAMTs were located at chromosome 13 and chromosome 1, respectively. Multiple alignment and phylogenetic analysis showed that JHAMT-1 was grouped together with insect JHAMTs independently and shared high similarities with insect JHAMTs compared to the other seven JHAMTs. In addition, JHAMT-1, JHAMT-2, and JHAMT-3 were highly expressed in the abdomen of spiderlings and could respond to the stimulation of exogenous farnesoic acid. Meanwhile, knockdown of these three JHAMTs caused the overweight and accelerated molting of spiderlings. These results demonstrated the cooperation of multi-JHAMTs in spider development and provided a new evolutionary perspective of the expansion of JHAMT in Arachnida. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1661-6596 1422-0067 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a1e3145bfa0387a26f947a94798a5bf8 https://www.mdpi.com/1422-0067/22/21/11721 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....a1e3145bfa0387a26f947a94798a5bf8 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16616596 14220067 |
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