Amsp3 may act upstream of Amdnmt3 in female caste differentiation in the honeybee ( Apis mellifera )
| العنوان: | Amsp3 may act upstream of Amdnmt3 in female caste differentiation in the honeybee ( |
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| المؤلفون: | Zilong Wang, M. Li, L.-X. Pan, F.-Y. Zhao, F.-P. Cheng |
| المصدر: | Insect Molecular Biology. 30:532-540 |
| بيانات النشر: | Wiley, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | food.ingredient, Biology, food, RNA interference, Royal jelly, Genetics, Animals, DNA (Cytosine-5-)-Methyltransferases, Molecular Biology, Gene, Transcription factor, Gene knockdown, fungi, Promoter, Bees, Phenotype, Juvenile Hormones, Sp3 Transcription Factor, Gene Knockdown Techniques, Larva, Insect Science, Juvenile hormone, Insect Proteins, Female, RNA Interference |
| الوصف: | In honey bees, the process of producing two female castes, including queens and workers, is nutritionally controlled by differential feeding royal jelly to newly emerged larvae. Although they have almost identical genetic blueprints, these castes show striking differences in their morphologies, longevities and reproductive capabilities. DNA methyltransferase 3 (Amdnmt3) gene is involved in the regulatory network for honeybee caste differentiation. Due to the role of two zinc fingers containing transcription factors, SP1 and SP3 in controlling mammalian Dnmts, this study aimed to determine a similar interaction of SPs with Amdnmt3 in the honeybee. We confirmed that the promoter region of Amdnmt3 contained multiple predicted SP1/SP3 binding sites and then investigated the role of AmSP3 in queen-worker differentiation network. We observed that the expression level of Amsp3 was significantly higher in worker larvae than that in queen larvae at 48 h, 84 h and 120 h. Knockdown of Amsp3 expression by RNAi in worker larvae significantly reduced the expression level of Amdnmt3 and caused morphological changes in adult bees towards a queen-like phenotype. However, the expression levels of Amsp3 and Amdnmt3 were repressed by juvenile hormone (JH). Our results suggest that AmSP3 is an important part of the queen-worker differentiation network and supports the role of Amdnmt3 in determining the phenotypic outcome of developing larvae. |
| تدمد: | 1365-2583 0962-1075 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a2370914d174a70bbc76ab1dd4d495c9 https://doi.org/10.1111/imb.12723 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....a2370914d174a70bbc76ab1dd4d495c9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13652583 09621075 |
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