Evaluation of Analytical Sensitivity of Sdf I based PCR and Sandwich ELISA for Salmonella Enteritidis detection and On-Farm prevalence in Punjab, Pakistan
العنوان: | Evaluation of Analytical Sensitivity of Sdf I based PCR and Sandwich ELISA for Salmonella Enteritidis detection and On-Farm prevalence in Punjab, Pakistan |
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المؤلفون: | MA Saeed, EH Syed, A Ghafor, T Yaqub, A Javeed, U Waheed |
المصدر: | Brazilian Journal of Poultry Science v.24 n.2 2022 Brazilian Journal of Poultry Science Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA) instacron:FACTA Brazilian Journal of Poultry Science, Volume: 24, Issue: 2, Article number: eRBCA-2021-1492, Published: 22 APR 2022 |
بيانات النشر: | FapUNIFESP (SciELO), 2022. |
سنة النشر: | 2022 |
مصطلحات موضوعية: | Analytical Sensitivity, animal diseases, Salmonella Enteritidis, Animal Science and Zoology, On-farm Prevalence |
الوصف: | Salmonella Enteritidis (SE) is a dominant serotype among non-typhoidal Salmonella which renders poultry products unsafe for human consumption. Due to frequent reporting of egg associated outbreaks, broiler breeder flocks are understudied although farm environment present supporting conditions for the growth of SE. In this study, two rapid detection techniques for SE were compared in terms of analytical sensitivity and the extent of SE contamination in broiler breeder farm environment was determined. Analytical sensitivity as limit of detection (LOD) was evaluated quantitatively for serotype specific PCR based on amplification of Sdf I gene and a commercially available sandwich ELISA for antigen detection. In triplicate experiments, tenfold serial dilutions of SE were prepared and tested with each technique. Using pure cultures, analytical sensitivity of PCR and ELISA were found to be 18.6 CFU/ml and 2.77×105 CFU/ml respectively. PCR (LOD, log 1.2) was found to be more sensitive and rapid than ELISA (LOD, log 5.4). Environmental swab samples (n = 260) were collected from 22 hen houses representing 8 broiler breeder farms located in and around Lahore and Sheikhupura districts of Punjab province. From each hen house swab samples were collected from litter, nests, feeders, drinkers, fans, pads, ceiling, walls and walkways. Following selective enrichment, pooled swab samples were subjected to PCR. Results showed that 36.3 % (8/22) hen houses were detected positive for SE. These findings suggest improvement in farm biosecurity measures and advocate implementation of integrated Salmonellosis control programs in broiler breeder houses to minimize carcass contamination. |
وصف الملف: | text/html |
تدمد: | 1806-9061 1516-635X |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a2ba5044f3c73913dfb40c75308ab8af https://doi.org/10.1590/1806-9061-2021-1492 |
حقوق: | OPEN |
رقم الأكسشن: | edsair.doi.dedup.....a2ba5044f3c73913dfb40c75308ab8af |
قاعدة البيانات: | OpenAIRE |
تدمد: | 18069061 1516635X |
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