PCR-based identification of Mycobacterium murphy causing Canine Leproid Granuloma Syndrome in Niterói, southeast Brazil - case report
| العنوان: | PCR-based identification of Mycobacterium murphy causing Canine Leproid Granuloma Syndrome in Niterói, southeast Brazil - case report |
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| المؤلفون: | V. Nowosh, R.C.S.M. Neves, A.N.B. Fontes, K.M.O. Silva, G.B. Queiroz, M.A.A. Pereira, A.C.P. Vicente, M.C.S. Lourenço, Philip Noel Suffys, S. Morgado |
| المصدر: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Vol 70, Iss 6, Pp 1699-1702 Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.70 n.6 2018 Arquivo brasileiro de medicina veterinária e zootecnia Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
| بيانات النشر: | Universidade Federal de Minas Gerais |
| مصطلحات موضوعية: | 0301 basic medicine, medicine.medical_specialty, 040301 veterinary sciences, 030106 microbiology, sequência hsp65, molecular methods, law.invention, isolado Metcalfe, 0403 veterinary science, 03 medical and health sciences, law, hsp65 sequence, medicine, Polymerase chain reaction, lcsh:SF1-1100, General Veterinary, biology, business.industry, isolate Metcalfe, virus diseases, 04 agricultural and veterinary sciences, medicine.disease, biology.organism_classification, Virology, CLGS, métodos moleculares, Infectious disease (medical specialty), Cytopathology, Granuloma, Histopathology, Leprosy, lcsh:Animal culture, Differential diagnosis, business, Mycobacterium |
| الوصف: | Canine Leproid Granuloma Syndrome (CLGS), also known as canine leprosy, is a cutaneous nodular infectious disease caused by Mycobacterium sp.. Despite being reported worldwide, it is still quite unknown and underdiagnosed. Diagnosis may be achieved by cytopathology or histopathology of skin lesions, but identification of the infectious agent is complex, since bacterial in vitro growth is not possible, relying upon molecular techniques such as PCR to confirm Mycobacterium DNA in the sample. We report a CLGS case in Niteroi, Rio de Janeiro state, Brazil, diagnosed by cytopathology and submitted to molecular identification of the agent. PCR amplification of hsp65 gene was performed and revealed 100% genetic homology to M. murphy strain. This is the first CLGS report with molecular identification in Rio de Janeiro state, and this finding should raise awareness about CLGS as a differential diagnosis among granulomatous skin diseases in this region. |
| وصف الملف: | text/html |
| اللغة: | English |
| تدمد: | 1678-4162 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a2e83277d3854b71887aa688857655d2 http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000601699&lng=en&tlng=en |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....a2e83277d3854b71887aa688857655d2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16784162 |
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