Study on the prevalence and genetic diversity of Eimeria species from broilers and free-range chickens in KwaZulu-Natal province, South Africa
| العنوان: | Study on the prevalence and genetic diversity of Eimeria species from broilers and free-range chickens in KwaZulu-Natal province, South Africa |
|---|---|
| المؤلفون: | Damer P. Blake, Samson Mukaratirwa, Oliver T. Zishiri, Sunday O. Peters, Matthew A. Adeleke, Abiodun J. Fatoba, Jeffrey Lebepe |
| المصدر: | Onderstepoort Journal of Veterinary Research, Volume: 87, Issue: 1, Pages: 1-10, Published: 2020 The Onderstepoort Journal of Veterinary Research Onderstepoort Journal of Veterinary Research, Vol 87, Iss 1, Pp e1-e10 (2020) |
| بيانات النشر: | ARC-Onderstepoort Veterinary Institute - Agricultural Research Council, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Veterinary medicine, animal diseases, prevalence, Eimeria brunetti, Eimeria, Feces, South Africa, parasitic diseases, molecular diagnosis, medicine, Animals, Phylogeny, Poultry Diseases, coccidiosis, Original Research, Genetic diversity, lcsh:Veterinary medicine, General Veterinary, biology, Coccidiosis, Oocysts, Free range, Broiler, Genetic Variation, food and beverages, General Medicine, genetic diversity, biology.organism_classification, medicine.disease, Eimeria acervulina, Eimeria maxima, lcsh:SF600-1100, chickens |
| الوصف: | This study was conducted from January to October 2018 with the objective to determine the prevalence and genetic diversity of Eimeria species in broiler and free-range chickens in KwaZulu-Natal province, South Africa. A total of 342 faecal samples were collected from 12 randomly selected healthy broiler chicken farms and 40 free-range chickens from 10 different locations. Faecal samples were screened for the presence of Eimeria oocysts using a standard flotation method. The species of Eimeria isolates were confirmed by amplification of the internal transcribed spacer 1 (ITS-1) partial region and sequences analysis. Among broiler and free-ranging chickens, 19 out of 41 pens (46.3%) and 25 out of 42 faecal samples (59.5%) were positive for Eimeria infection. Molecular detection revealed the following species: Eimeria maxima, Eimeria tenella, Eimeria acervulina, Eimeria brunetti and Eimeria mitis in all the samples screened. Similarly, polymerase chain reaction assays specific for three cryptic Eimeria operational taxonomic units were negative for all the samples. Phylogenetic analysis of the ITS-1 sequences supported species identity with the greatest variation detected for E. mitis. This study provides information on the range and identity of Eimeria species, and their genetic relatedness, circulating in commercially reared broilers and free-ranging chickens from different locations in KwaZulu-Natal province. |
| وصف الملف: | text/html |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a2ee9ca91fa6ace2746eb212427b5106 http://www.scielo.org.za/scielo.php?script=sci_arttext&pid=S0030-24652020000100010&lng=en&tlng=en |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....a2ee9ca91fa6ace2746eb212427b5106 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |