Short-Chain Fatty Acids (Except Hexanoic Acid) Lower NF-kB Transactivation, Which Rescues Inflammation-Induced Decreased Apolipoprotein A-I Transcription in HepG2 Cells
| العنوان: | Short-Chain Fatty Acids (Except Hexanoic Acid) Lower NF-kB Transactivation, Which Rescues Inflammation-Induced Decreased Apolipoprotein A-I Transcription in HepG2 Cells |
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| المؤلفون: | Herman E. Popeijus, Fatma B. A. Mokhtar, Jehad Z. Tayyeb, Jogchum Plat, Maurice C. J. M. Konings, Ronald P. Mensink |
| المساهمون: | Nutrition and Movement Sciences, RS: NUTRIM - R1 - Obesity, diabetes and cardiovascular health |
| المصدر: | International journal of molecular sciences, 21(14):5088. Multidisciplinary Digital Publishing Institute (MDPI) International Journal of Molecular Sciences, Vol 21, Iss 5088, p 5088 (2020) International Journal of Molecular Sciences Volume 21 Issue 14 |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, SCFAs, Apolipoprotein B, Proto-Oncogene Proteins c-jun, PPARα, NF-κB, lcsh:Chemistry, ACTIVATION, chemistry.chemical_compound, Transactivation, 0302 clinical medicine, Transcription (biology), PPAR-ALPHA, polycyclic compounds, lcsh:QH301-705.5, Spectroscopy, GENE-EXPRESSION, Kelch-Like ECH-Associated Protein 1, biology, Chemistry, ApoA-I, GUT MICROBIOTA, NF-kappa B, Hep G2 Cells, General Medicine, Computer Science Applications, Butyrates, FACTOR-KAPPA-B, 030220 oncology & carcinogenesis, I-kappa B Proteins, lipids (amino acids, peptides, and proteins), medicine.symptom, Proto-Oncogene Proteins c-fos, Transcriptional Activation, medicine.medical_specialty, INHIBITION, Inflammation, Butyrate, Article, Catalysis, Proinflammatory cytokine, Inorganic Chemistry, 03 medical and health sciences, Carnitine palmitoyltransferase 1, BUTYRATE, Internal medicine, Valerates, medicine, Humans, PPAR alpha, Physical and Theoretical Chemistry, Caproates, Molecular Biology, Apolipoprotein A-I, Carnitine O-Palmitoyltransferase, Interleukin-8, Organic Chemistry, nutritional and metabolic diseases, Fatty Acids, Volatile, 030104 developmental biology, Endocrinology, lcsh:Biology (General), lcsh:QD1-999, inflammation, biology.protein, Propionates |
| الوصف: | Concentrations of apolipoprotein A-I (ApoA-I) decrease during inflammation, which may lead to dysfunctional ApoA-I-poor high-density lipoprotein (HDL) particles, and as such, elevate cardiovascular risk. Therefore, rescuing ApoA-I concentrations, especially during inflammation, seems beneficial. Recently, short-chain fatty acids (SCFAs) have received more attention as a strategy in reversing atherosclerosis. We here evaluated the effects of SCFAs on inflammatory pathways in relation to ApoA-I transcription. SCFAs dose&ndash response studies were performed in the presence and absence of inflammatory cytokines. ApoA-I and interleukin 8 (IL-8) mRNA expression were analyzed using qPCR and ELISA, respectively. To study underlying mechanisms, nuclear factor kappa B (NF-&kappa B) transactivation and changes in mRNA expressions of the genes targets of bromodomain and extra-terminal (BET) inhibition, peroxisome proliferator-activated receptor-alpha (PPAR&alpha ) transactivation and activator protein 1 (AP-1) pathway were analyzed. SCFAs (except hexanoic acid) increased ApoA-I mRNA transcription in both normal and inflammatory conditions and lowered IL-8 mRNA expression. This anti-inflammatory effect of SCFAs was confirmed by inhibition of NF-&kappa B transactivation. Moreover, butyric acid increased carnitine palmitoyltransferase 1 (CPT1), PPAR&alpha target gene, mRNA transcription in both conditions, and there was a negative correlation between CPT1 and NF-&kappa B. Therefore, PPAR&alpha transactivation is probably involved in the anti-inflammatory effects of SCFAs, which rescues ApoA-I transcription. In conclusion, propionate, butyrate and valerate elicit anti-inflammatory effects which might rescue ApoA-I transcription in inflammatory conditions via PPAR&alpha transactivation mediated NF-&kappa B inhibition. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1661-6596 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a30f0388fbb415bd9605f332214c49d0 https://doi.org/10.3390/ijms21145088 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....a30f0388fbb415bd9605f332214c49d0 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16616596 |
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