Graphene oxide accelerates diabetic wound repair by inhibiting apoptosis of Ad‐MSCs via Linc00324/ miR ‐7977/ STK4 pathway
| العنوان: | Graphene oxide accelerates diabetic wound repair by inhibiting apoptosis of |
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| المؤلفون: | Zhe Ji, Jian Wang, Shuai Yang, Shengjun Tao, Caiqi Shen, Hanxiao Wei, Qiang Li, Peisheng Jin |
| المصدر: | The FASEB Journal. 36 |
| بيانات النشر: | Wiley, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Wound Healing, Apoptosis, Mesenchymal Stem Cells, Protein Serine-Threonine Kinases, Biochemistry, MicroRNAs, Glucose, Diabetes Mellitus, Genetics, Humans, RNA, Long Noncoding, Graphite, Luciferases, Molecular Biology, Biotechnology |
| الوصف: | Many studies have shown that graphene oxide (GO) promotes proliferation and differentiation of a variety of stem cells. However, its effect on adipose-derived mesenchymal stem cell (Ad-MSCs) apoptosis is still unclear. Apoptosis is a significant factor affecting stem cell-based treatment of diabetic wounds. Therefore, we explored the effect of GO on Ad-MSC apoptosis and diabetic wound healing. In this study, qRT-PCR was used to detect Ad-MSC expression of LncRNAs, miRNAs, and mRNAs under high-glucose environment. RNA immunoprecipitation (RIP), RNA pull-down, and luciferase assays were used to detect interactions of specific lncRNAs, miRNAs, and mRNAs. The effects of GO on Ad-MSC apoptosis were explored by flow cytometry, TUNEL assay, and Western blot. A diabetic wound model was used to explore the function of Linc00324 on Ad-MSC reparative properties in vivo. As a result, GO inhibited high glucose-induced apoptosis in Ad-MSCs, and Linc00324 contributed to the anti-apoptotic effect of GO. RIP and RNA pull-down confirmed that Linc00324 directly interacted with miR-7977, functioning as a miRNA sponge to regulate expression of the miR-7977 target gene STK4 (MST1) and downstream signaling pathways. In addition, GO reduced the apoptosis of Ad-MSCs in wounds and promoted wound healing. Taken together, these findings suggest GO may be a superior auxiliary material for Ad-MSCs to facilitate diabetic wound healing via the Linc00324/miR-7977/STK4 pathway. |
| تدمد: | 1530-6860 0892-6638 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a31353a8e28202007fa725501db45d32 https://doi.org/10.1096/fj.202201079rr |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....a31353a8e28202007fa725501db45d32 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15306860 08926638 |
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