Daily rhythms in gene expression of the human parasite Schistosoma mansoni
| العنوان: | Daily rhythms in gene expression of the human parasite Schistosoma mansoni |
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| المؤلفون: | Matthew Berriman, Karl F. Hoffmann, Zhigang Lu, Patrick Driguez, Carmen Diaz Soria, Nancy Holroyd, Avril Coghlan, David C. Wilcockson, Mandy Sanders, Gabriel Rinaldi, Anna Wawer, Kate A. Rawlinson, Geetha Sankaranarayanan, Catherine McCarthy, Adam J. Reid, Sarah K. Buddenborg |
| المصدر: | BMC Biology, Vol 19, Iss 1, Pp 1-21 (2021) BMC Biology |
| بيانات النشر: | BMC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Male, Physiology, QH301-705.5, Circadian clock, Plant Science, Biology, Daily rhythms, General Biochemistry, Genetics and Molecular Biology, Mice, 03 medical and health sciences, 0302 clinical medicine, Rhythm, Structural Biology, Circadian Clocks, Animals, Humans, Parasites, Biology (General), Transcriptomics, Gene, Ecology, Evolution, Behavior and Systematics, 030304 developmental biology, 0303 health sciences, Chronobiology, Host (biology), Schistosoma mansoni, Cell Biology, biology.organism_classification, Circadian Rhythm, 3. Good health, Adult Schistosoma mansoni, CLOCK, Evolutionary biology, Human parasite, Female, RNA-seq, Animal circadian clock genes, Transcriptome, General Agricultural and Biological Sciences, 030217 neurology & neurosurgery, Research Article, Developmental Biology, Biotechnology |
| الوصف: | Background The consequences of the earth’s daily rotation have led to 24-h biological rhythms in most organisms. Even some parasites are known to have daily rhythms, which, when in synchrony with host rhythms, can optimise their fitness. Understanding these rhythms may enable the development of control strategies that take advantage of rhythmic vulnerabilities. Recent work on protozoan parasites has revealed 24-h rhythms in gene expression, drug sensitivity and the presence of an intrinsic circadian clock; however, similar studies on metazoan parasites are lacking. To address this, we investigated if a metazoan parasite has daily molecular oscillations, whether they reveal how these longer-lived organisms can survive host daily cycles over a lifespan of many years and if animal circadian clock genes are present and rhythmic. We addressed these questions using the human blood fluke Schistosoma mansoni that lives in the vasculature for decades and causes the tropical disease schistosomiasis. Results Using round-the-clock transcriptomics of male and female adult worms collected from experimentally infected mice, we discovered that ~ 2% of its genes followed a daily pattern of expression. Rhythmic processes included a stress response during the host’s active phase and a ‘peak in metabolic activity’ during the host’s resting phase. Transcriptional profiles in the female reproductive system were mirrored by daily patterns in egg laying (eggs are the main drivers of the host pathology). Genes cycling with the highest amplitudes include predicted drug targets and a vaccine candidate. These 24-h rhythms may be driven by host rhythms and/or generated by a circadian clock; however, orthologs of core clock genes are missing and secondary clock genes show no 24-h rhythmicity. Conclusions There are daily rhythms in the transcriptomes of adult S. mansoni, but they appear less pronounced than in other organisms. The rhythms reveal temporally compartmentalised internal processes and host interactions relevant to within-host survival and between-host transmission. Our findings suggest that if these daily rhythms are generated by an intrinsic circadian clock then the oscillatory mechanism must be distinct from that in other animals. We have shown which transcripts oscillate at this temporal scale and this will benefit the development and delivery of treatments against schistosomiasis. |
| اللغة: | English |
| تدمد: | 1741-7007 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a393f1fd27fe79ad9c98665828b23d5f https://doaj.org/article/9c514beb10074835b2b16a2af319d0f5 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....a393f1fd27fe79ad9c98665828b23d5f |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 17417007 |
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