Bioactivation routes of gelatin-based scaffolds to enhance at nanoscale level bone tissue regeneration
| العنوان: | Bioactivation routes of gelatin-based scaffolds to enhance at nanoscale level bone tissue regeneration |
|---|---|
| المؤلفون: | Luigi Ambrosio, Ugo D'Amora, Alfredo Ronca, Maria Grazia Raucci, Christian Demitri |
| المساهمون: | Raucci, M. G., D'Amora, U., Ronca, A., Demitri, C., Ambrosio, L. |
| المصدر: | Frontiers in Bioengineering and Biotechnology Frontiers in Bioengineering and Biotechnology, Vol 7 (2019) Frontiers in Bioengineering and Biotechnology 7 (2019). doi:10.3389/fbioe.2019.00027 info:cnr-pdr/source/autori:Raucci M.G.; D'Amora U.; Ronca A.; Demitri C.; Ambrosio L./titolo:Bioactivation routes of gelatin-based scaffolds to enhance at nanoscale level bone tissue regeneration/doi:10.3389%2Ffbioe.2019.00027/rivista:Frontiers in Bioengineering and Biotechnology/anno:2019/pagina_da:/pagina_a:/intervallo_pagine:/volume:7 |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Scaffold, Histology, food.ingredient, lcsh:Biotechnology, Biomedical Engineering, Bioengineering, 02 engineering and technology, scaffold, Bone tissue, Gelatin, Hydroxyapatite, 03 medical and health sciences, food, Bone tissue engineering and regeneration, lcsh:TP248.13-248.65, medicine, BMP-2 peptide, Original Research, natural polymer, Chemistry, Regeneration (biology), Mesenchymal stem cell, Bioengineering and Biotechnology, hydroxyapatite, Adhesion, 021001 nanoscience & nanotechnology, Natural polymer, 030104 developmental biology, medicine.anatomical_structure, bone tissue engineering and regeneration, Biophysics, Alkaline phosphatase, Surface modification, 0210 nano-technology, Biotechnology |
| الوصف: | The present work is focused on the development of gelatin-based scaffolds crosslinked through carbodiimide reaction and their bioactivation by two different methods: (i) surface modification by inorganic signals represented by hydroxyapatite nanoparticles precipitated on scaffold through biomimetic treatment; (ii) analog of BMP-2 peptide decoration. The results showed the effects of polymer concentration and crosslinking time on the physico-chemical, morphological, and mechanical properties of scaffolds. Furthermore, a comparative study of biological response for both bioactivated structures allowed to evaluate the influence of inorganic and organic cues on cellular behavior in terms of adhesion, proliferation and early osteogenic marker expression. The bioactivation by inorganic cues induced positive cellular response compared to neat scaffolds in terms of increased cell proliferation and early osteogenic differentiation of human mesenchymal stem cell (hMSC), as evidenced by the Alkaline phosphatase (ALP) expression. Similarly BMP-2 peptide decorated scaffolds showed higher values of ALP than biomineralized ones at longer time. The overall results demonstrated that the presence of bioactive signals (either inorganic or organic) at nanoscale level allowed an osteoinductive effect on hMSC in a basal medium, making the modified gelatin scaffolds a promising candidate for bone tissue regeneration. |
| وصف الملف: | ELETTRONICO |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a3b4bafa4c475000d940c6facf8ceda3 https://hdl.handle.net/11587/439181 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....a3b4bafa4c475000d940c6facf8ceda3 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |