A New Subclade of Leptosphaeria biglobosa Identified from Brassica rapa
| العنوان: | A New Subclade of Leptosphaeria biglobosa Identified from Brassica rapa |
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| المؤلفون: | W. G. Dilantha Fernando, Zhongwei Zou, Angela P. Van de Wouw, Paula Parks, Lindsey J. du Toit, Xuehua Zhang |
| المصدر: | International Journal of Molecular Sciences Volume 20 Issue 7 International Journal of Molecular Sciences, Vol 20, Iss 7, p 1668 (2019) |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Brassica juncea, Blackleg, Brassica oleracea, 01 natural sciences, australensis, Catalysis, Brassica napus, lcsh:Chemistry, Inorganic Chemistry, 03 medical and health sciences, Leptosphaeria maculans, Leptosphaeria biglobosa, Brassica rapa, Botany, erysimii, Physical and Theoretical Chemistry, Internal transcribed spacer, lcsh:QH301-705.5, Molecular Biology, Ribosomal DNA, subclades, Spectroscopy, biology, Phylogenetic tree, Organic Chemistry, food and beverages, Subclade, General Medicine, ITS rDNA, biology.organism_classification, brassicae, 3. Good health, Computer Science Applications, 030104 developmental biology, lcsh:Biology (General), lcsh:QD1-999, thlaspii, occiaustralensis, β-tubulin, actin, 010606 plant biology & botany, canadensis |
| الوصف: | Blackleg (Phoma stem canker) of crucifers is a globally important disease caused by the ascomycete species complex comprising of Leptosphaeria maculans and Leptosphaeria biglobosa. Six blackleg isolates recovered from Brassica rapa cv. Mizspoona in the Willamette Valley of Oregon were characterized as L. biglobosa based on standard pathogenicity tests and molecular phylogenetic analysis. These isolates were compared to 88 characterized L. biglobosa isolates from western Canada, 22 isolates from Australia, and 6 L. maculans isolates from Idaho, USA using maximum parsimony and distance analysis of phylogenetic trees generated from the ITS rDNA (internal transcribed spacer rDNA) sequence, and the actin and &beta tubulin gene sequences. The L. biglobosa isolates derived from B. rapa collected in Oregon formed a separate subclade based on concatenated gene sequences or a single gene sequence, regardless of the analyses. Pathogenicity tests showed that these isolates failed to infect either resistant or susceptible B. napus cultivars, but caused severe symptoms on three B. rapa cultivars (Accession number: UM1113, UM1112, and UM1161), a B. oleracea var. capitata (cabbage) cultivar (Copenhagen Market), and two B. juncea cultivars (CBM, a common brown Mustard, and Forge). These findings demonstrated that the L. biglobosa isolates derived from a B. rapa crop in Oregon were genetically distinct from existing species of L. biglobosa, and constitute a new subclade, herein proposed as L. biglobosa &lsquo americensis&rsquo |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1422-0067 |
| DOI: | 10.3390/ijms20071668 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a4b422d26fc55120fdb8842ca98c2cf7 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....a4b422d26fc55120fdb8842ca98c2cf7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14220067 |
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| DOI: | 10.3390/ijms20071668 |