The hepatoprotective effect of putrescine against cadmium-induced acute liver injury
| العنوان: | The hepatoprotective effect of putrescine against cadmium-induced acute liver injury |
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| المؤلفون: | Georgios I Panoutsopoulos, Kalliopi T. Kourentzi, George K. Papadimas, Michael G. Mykoniatis, Vasiliki G. Kondyli, Rosa I. Hereti, Maria D. Demonakou, Konstantinos N. Tzirogiannis |
| المصدر: | Archives of Toxicology. 78:321-329 |
| بيانات النشر: | Springer Science and Business Media LLC, 2004. |
| سنة النشر: | 2004 |
| مصطلحات موضوعية: | Male, Thymidine kinase activity, medicine.medical_specialty, Pathology, Programmed cell death, Necrosis, Health, Toxicology and Mutagenesis, Apoptosis, Biology, Protective Agents, Toxicology, Drug Administration Schedule, chemistry.chemical_compound, Internal medicine, Putrescine, Toxicity Tests, Acute, medicine, Animals, Aspartate Aminotransferases, Peliosis Hepatis, Rats, Wistar, Liver injury, Dose-Response Relationship, Drug, Alanine Transaminase, General Medicine, medicine.disease, Liver regeneration, Liver Regeneration, Rats, Endocrinology, Liver, chemistry, Hepatic stellate cell, Peliosis hepatis, Chemical and Drug Induced Liver Injury, medicine.symptom, Cadmium |
| الوصف: | The hepatoprotective effect of putrescine against cadmium liver injury was investigated. Male Wistar rats were injected with a dose of cadmium (6.5 mg CdCl(2)/kg bodyweight, intraperitoneally). Normal saline (group I) or putrescine (300 micro mol/kg bodyweight; group II) were injected 2, 5 and 8 h later. A number of animals of both groups were killed 0, 12, 16, 24, 48 or 60 h after cadmium intoxication. Liver tissue was histologically assessed for necrosis, apoptosis, peliosis, mitoses, and inflammatory infiltration. Apoptosis was also quantified by the TUNEL assay for hepatocytes and nonparenchymal liver cells. The discrimination between hepatic cell subpopulations was achieved histochemically. The mitotic index in hematoxylin-eosin-stained sections and by the immunochemical detection of Ki67 nuclear antigen, (3)H-thymidine incorporation into hepatic DNA, and hepatic thymidine kinase activity were all used as indices of liver regeneration. Both hepatocyte apoptosis and liver necrosis evolved in a biphasic temporal pattern. Nonparenchymal cell apoptosis and peliosis hepatis evolved in a monophasic pattern and were correlated closely. Putrescine administration totally reversed liver necrosis and hepatocyte apoptosis. The time profile of nonparenchymal apoptosis was altered and peliosis hepatis was also totally attenuated. In conclusion, putrescine protected hepatocytes and modulated the mechanism of cadmium-induced acute hepatotoxicity. |
| تدمد: | 1432-0738 0340-5761 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a578c938babb94ce508de3018756859d https://doi.org/10.1007/s00204-004-0549-0 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....a578c938babb94ce508de3018756859d |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14320738 03405761 |
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