Luminescent Amphiphilic Aminoglycoside Probes to Study Transfection
| العنوان: | Luminescent Amphiphilic Aminoglycoside Probes to Study Transfection |
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| المؤلفون: | Johannes Koch, Micha Fridman, Qais Z. Jaber, Cecilia Vallet, Alexander Zimmermann, Steffen Riebe, Shirley K. Knauer, Kfir B. Steinbuch, Jens Voskuhl, Matthias Hayduk, Kateryna Loza |
| المصدر: | Chembiochem |
| بيانات النشر: | John Wiley and Sons Inc., 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | aggregation-induced emission, Cell Survival, Static Electricity, Chemie, 010402 general chemistry, Transfection, 01 natural sciences, Biochemistry, Green fluorescent protein, HeLa, chemistry.chemical_compound, Amphiphile, Animals, Humans, bioimaging, Molecular Biology, Microscopy, Confocal, biology, 010405 organic chemistry, Chemistry, Communication, Organic Chemistry, Cationic polymerization, self-assembly, transfection agents, biology.organism_classification, Lipids, Communications, 0104 chemical sciences, Aminoglycosides, HEK293 Cells, Reagent, cationic amphiphiles, Biophysics, Luminophore, Tobramycin, Molecular Medicine, Self-assembly, Biologie, HeLa Cells, Plasmids |
| الوصف: | We report the characterization of amphiphilic aminoglycoside conjugates containing luminophores with aggregation‐induced emission properties as transfection reagents. These inherently luminescent transfection vectors are capable of binding plasmid DNA through electrostatic interactions; this binding results in an emission “on” signal due to restriction of intramolecular motion of the luminophore core. The luminescent cationic amphiphiles effectively transferred plasmid DNA into mammalian cells (HeLa, HEK 293T), as proven by expression of a red fluorescent protein marker. The morphologies of the aggregates were investigated by microscopy as well as ζ‐potential and dynamic light‐scattering measurements. The transfection efficiencies using luminescent cationic amphiphiles were similar to that of the gold‐standard transfection reagent Lipofectamine® 2000. Transfection tracker: Conjugates of luminophores with aggregation‐induced emission properties linked to the aminoglycoside tobramycin are reported. These compounds reveal an emission “on” behavior upon plasmid‐DNA binding and are able to transfect different mammalian cell lines. The cellular uptake was investigated using the emission properties of the formed plasmid DNA‐vector assemblies. |
| اللغة: | English |
| تدمد: | 1439-7633 1439-4227 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a583973f9a6b1f427fd5412384bd90e2 http://europepmc.org/articles/PMC8248372 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....a583973f9a6b1f427fd5412384bd90e2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14397633 14394227 |
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