Unpacking the aggregation-oligomerization-fibrillization process of naturally-occurring hIAPP amyloid oligomers isolated directly from sera of children with obesity or diabetes mellitus
| العنوان: | Unpacking the aggregation-oligomerization-fibrillization process of naturally-occurring hIAPP amyloid oligomers isolated directly from sera of children with obesity or diabetes mellitus |
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| المؤلفون: | Luis Felipe Jiménez-García, Edgar Leyva-García, Reyna Lara-Martínez, Perla Altamirano, Myriam M. Altamirano-Bustamante, Eulalia P. Garrido-Magaña, Cristina Revilla-Monsalve, Mateo Larralde-Laborde, Raúl Calzada-León, Nelly F. Altamirano-Bustamante, Gerardo Rojas |
| المصدر: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-14 (2019) |
| بيانات النشر: | Nature Publishing Group UK, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, Amyloid, lcsh:Medicine, Fibril, Protein Aggregation, Pathological, Article, 03 medical and health sciences, Protein Aggregates, 0302 clinical medicine, Diabetes mellitus, Insulin-Secreting Cells, medicine, Humans, Obesity, lcsh:Science, Child, geography, Multidisciplinary, geography.geographical_feature_category, Chemistry, lcsh:R, Type 2 diabetes, medicine.disease, Islet, Islet Amyloid Polypeptide, 030104 developmental biology, Diabetes Mellitus, Type 1, Biochemistry, Diabetes Mellitus, Type 2, lcsh:Q, Female, Protein aggregation, Protein Multimerization, 030217 neurology & neurosurgery |
| الوصف: | The formation of amyloid oligomers and fibrils of the human islet amyloid polypeptide (hIAPP) has been linked with β- cell failure and death which causes the onset, progression, and comorbidities of diabetes. We begin to unpack the aggregation-oligomerization-fibrillization process of these oligomers taken from sera of pediatric patients. The naturally occurring or real hIAPP (not synthetic) amyloid oligomers (RIAO) were successfully isolated, we demonstrated the presence of homo (dodecamers, hexamers, and trimers) and hetero-RIAO, as well as several biophysical characterizations which allow us to learn from the real phenomenon taking place. We found that the aggregation/oligomerization process is active in the sera and showed that it happens very fast. The RIAO can form fibers and react with anti-hIAPP and anti-amyloid oligomers antibodies. Our results opens the epistemic horizon and reveal real differences between the four groups (Controls vs obesity, T1DM or T2DM) accelerating the process of understanding and discovering novel and more efficient prevention, diagnostic, transmission and therapeutic pathways. |
| اللغة: | English |
| تدمد: | 2045-2322 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a61c345e0bbf9bbacce34dd1ced92d59 http://europepmc.org/articles/PMC6895187 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....a61c345e0bbf9bbacce34dd1ced92d59 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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