Streptococcus salivarius inhibits immune activation by periodontal disease pathogens
| العنوان: | Streptococcus salivarius inhibits immune activation by periodontal disease pathogens |
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| المؤلفون: | Ryan M. Chanyi, Kyle MacDonald, Peter A. Cadieux, Jeremy P. Burton, Gregor Reid, Jean M. Macklaim |
| المصدر: | BMC Oral Health BMC Oral Health, Vol 21, Iss 1, Pp 1-16 (2021) |
| بيانات النشر: | Springer Science and Business Media LLC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | medicine.medical_treatment, Aggregatibacter actinomycetemcomitans, Streptococcus salivarius, law.invention, Proinflammatory cytokine, Microbiology, 03 medical and health sciences, Probiotic, stomatognathic system, Immune inhibition, law, Humans, Medicine, Microbiome, General Dentistry, Porphyromonas gingivalis, Periodontal Diseases, 030304 developmental biology, 0303 health sciences, Fusobacterium nucleatum, biology, 030306 microbiology, business.industry, Probiotics, RK1-715, biology.organism_classification, Chewing gum, stomatognathic diseases, Cytokine, Dentistry, bacteria, Periodontal disease, business, Research Article |
| الوصف: | Background Periodontal disease represents a major health concern. The administration of beneficial microbes has been increasing in popularity over efforts to manipulate the microbes using antimicrobial agents. This study determined the ability of Streptococcus salivarius to inhibit IL-6 and IL-8 production by gingival fibroblasts when activated by periodontal pathogens and their effect on the salivary microbiome. Methods Primary human gingival fibroblasts were challenged with Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans and Fusobacterium nucleatum and a combination of all three. IL-6 and IL-8 cytokine release were measured. Using this same model, S. salivarius K12, M18 and different supernatant and whole-cell lysate fractions of S. salivarius K12 were administered to pathogen-induced fibroblasts. A patient study of healthy participants was also conducted to determine the effect S. salivarius K12 had on the native microbiome using 16S next generation sequence analysis. Results All pathogens tested induced a significant IL-6 and IL-8 response. S. salivarius K12 or M18, did not exhibit an increase in inflammatory cytokines. When either of the probiotic strains were co-administered with a pathogen, there were significant reductions in both IL-6 and IL-8 release. This effect was also observed when gingival fibroblasts were pre-treated with either S. salivarius K12 or M18 and then stimulated with the oral pathogens. Chewing gum containing S. salivarius K12 did not alter the salivary microbiome and did not increase inflammatory markers in the oral cavity. Conclusion S. salivarius K12 and M18 prevented immune activation induced by periodontal disease pathogens. S. salivarius K12 did not alter the salivary microbiome or induce immune activation when administered as a chewing gum. These results warrant further study to determine if it may be an effective treatment in a model of periodontal disease. |
| تدمد: | 1472-6831 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a64fb99c9a41b24f05cdd379c8e792f3 https://doi.org/10.1186/s12903-021-01606-z |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....a64fb99c9a41b24f05cdd379c8e792f3 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14726831 |
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