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Transcriptome response comparison between vector and non-vector aphids after feeding on virus-infected wheat plants

التفاصيل البيبلوغرافية
العنوان: Transcriptome response comparison between vector and non-vector aphids after feeding on virus-infected wheat plants
المؤلفون: Gaisheng Zhang, Shize Zhang, Zeqian Tong, Chi Zhang, Dan Su, Zuqing Hu, Huiyan Zhao, Dandan Li
المصدر: BMC Genomics, Vol 21, Iss 1, Pp 1-14 (2020)
BMC Genomics
سنة النشر: 2020
مصطلحات موضوعية: BYDV, lcsh:QH426-470, lcsh:Biotechnology, UniGene, Gene clone, Biology, Virus, Transcriptome response, Transcriptome, Rhopalosiphum padi, Plant virus, lcsh:TP248.13-248.65, Gene expression, Luteovirus, Genetics, Wheat dwarf virus, STAT5 Transcription Factor, Animals, Triticum, Janus Kinases, WDV, food and beverages, biochemical phenomena, metabolism, and nutrition, biology.organism_classification, Insect Vectors, lcsh:Genetics, Geminiviridae, Barley yellow dwarf, Aphids, Non-vector, Dicistroviridae, Insect Proteins, Vector, Research Article, Biotechnology
الوصف: Background Plant viruses maintain intricate interactions with their vector and non-vector insects and can impact the fitness of insects. However, the details of their molecular and cellular mechanisms have not been studied well. We compared the transcriptome-level responses in vector and non-vector aphids (Schizaphis graminum and Rhopalosiphum padi, respectively) after feeding on wheat plants with viral infections (Barley Yellow Dwarf Virus (BYDV) and Wheat dwarf virus (WDV), respectively). We conducted differentially expressed gene (DEG) annotation analyses and observed DEGs related to immune pathway, growth, development, and reproduction. And we conducted cloning and bioinformatic analyses of the key DEG involved in immune. Results For all differentially expressed gene analyses, the numbers of DEGs related to immune, growth, development, reproduction and cuticle were higher in vector aphids than in non-vector aphids. STAT5B (signal transducer and activator of transcription 5B), which is involved in the JAK-STAT pathway, was upregulated in R. padi exposed to WDV. The cloning and bioinformatic results indicated that the RpSTAT5B sequence contains a 2082 bp ORF encoding 693 amino acids. The protein molecular weight is 79.1 kD and pI is 8.13. Analysis indicated that RpSTAT5B is a non-transmembrane protein and a non-secreted protein. Homology and evolutionary analysis indicated that RpSTAT5B was closely related to R. maidis. Conclusions Unigene expression analysis showed that the total number of differentially expressed genes (DEGs) in the vector aphids was higher than that in the non-vector aphids. Functional enrichment analysis showed that the DEGs related to immunity, growth and reproduction in vector aphids were higher than those in non-vector aphids, and the differentially expressed genes related to immune were up-regulated. This study provides a basis for the evaluation of the response mechanisms of vector/non-vector insects to plant viruses.
تدمد: 1471-2164
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a66f68a1dc837f4f569981e904593291
https://pubmed.ncbi.nlm.nih.gov/32933469
حقوق: OPEN
رقم الأكسشن: edsair.doi.dedup.....a66f68a1dc837f4f569981e904593291
قاعدة البيانات: OpenAIRE
الوصف
تدمد:14712164