In-depth characterization of the cerebrospinal fluid (CSF) proteome displayed through the CSF proteome resource (CSF-PR)
| العنوان: | In-depth characterization of the cerebrospinal fluid (CSF) proteome displayed through the CSF proteome resource (CSF-PR) |
|---|---|
| المؤلفون: | Hilde Garberg, Heidrun Vethe, Jill A. Opsahl, Magnus Berle, Frode S. Berven, Eystein Oveland, Yehia Farag, Harald Barsnes, Astrid Guldbrandsen, Kjell-Morten Myhr |
| المصدر: | Molecular & Cellular Proteomics Molecular & Cellular Proteomics : MCP |
| بيانات النشر: | ASBMB, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Resource, Proteomics, Glycosylation, Proteome, Midical sciences: 700::Clinical medical sciences: 750::Neurology: 752 [VDP], Quantitative proteomics, Biology, Tandem mass spectrometry, Biochemistry, Analytical Chemistry, chemistry.chemical_compound, Cerebrospinal fluid, Tandem Mass Spectrometry, Humans, Molecular Biology, Cerebrospinal Fluid, Glycoproteins, chemistry.chemical_classification, Gene Expression Profiling, Glycopeptides, Molecular biology, Healthy Volunteers, Glycopeptide, chemistry, Medisinske fag: 700::Klinisk medisinske fag: 750::Nevrologi: 752 [VDP], Electrophoresis, Polyacrylamide Gel, Glycoprotein, Chromatography, Liquid |
| الوصف: | In this study, the human cerebrospinal fluid (CSF) proteome was mapped using three different strategies prior to Orbitrap LC-MS/MS analysis: SDS-PAGE and mixed mode reversed phase-anion exchange for mapping the global CSF proteome, and hydrazide-based glycopeptide capture for mapping glycopeptides. A maximal protein set of 3081 proteins (28,811 peptide sequences) was identified, of which 520 were identified as glycoproteins from the glycopeptide enrichment strategy, including 1121 glycopeptides and their glycosylation sites. To our knowledge, this is the largest number of identified proteins and glycopeptides reported for CSF, including 417 glycosylation sites not previously reported. From parallel plasma samples, we identified 1050 proteins (9739 peptide sequences). An overlap of 877 proteins was found between the two body fluids, whereas 2204 proteins were identified only in CSF and 173 only in plasma. All mapping results are freely available via the new CSF Proteome Resource (http://probe.uib.no/csf-pr), which can be used to navigate the CSF proteome and help guide the selection of signature peptides in targeted quantitative proteomics. publishedVersion |
| وصف الملف: | application/pdf |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a6c4591af3916777e1c7ff0eadf47d97 https://hdl.handle.net/1956/16685 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....a6c4591af3916777e1c7ff0eadf47d97 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |