Dependence of Aortic Arch Morphogenesis on Intracardiac Blood Flow in the Left Atrial Ligated Chick Embryo
العنوان: | Dependence of Aortic Arch Morphogenesis on Intracardiac Blood Flow in the Left Atrial Ligated Chick Embryo |
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المؤلفون: | Amit Agrawal, Edward B. Clark, Douglas A. Christensen, John A. Hawkins, Norman Hu, Charity Beaumont |
المصدر: | The Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology. 292:652-660 |
بيانات النشر: | Wiley, 2009. |
سنة النشر: | 2009 |
مصطلحات موضوعية: | Heart Defects, Congenital, Models, Anatomic, Aortic arch, medicine.medical_specialty, Histology, Systole, Aorta, Thoracic, Chick Embryo, Vitelline veins, Biology, Functional Laterality, Intracardiac injection, Suture (anatomy), Diastole, Internal medicine, medicine.artery, medicine, Animals, Heart Atria, cardiovascular diseases, Ligation, Ecology, Evolution, Behavior and Systematics, Aorta, Aortic Arch Syndromes, Stroke volume, Blood flow, Anatomy, Regional Blood Flow, Ultrasonography, Doppler, Pulsed, cardiovascular system, Cardiology, Tomography, X-Ray Computed, Biotechnology |
الوصف: | Partial left atrial ligation before cardiac septation redistributes intracardiac blood flow and produces left ventricular hypoplasia in the chick. We hypothesized that redistributed intracardiac blood flow adversely alters aortic arch development. We ligated the left atrial appendage with a 10-0 nylon suture at stage 21 chick embryos, then reincubated up to stage 34. Sham embryos had a suture tied adjacent to the atrial wall, and normal controls were unoperated. We measured simultaneous atrioventricular (AV) and dorsal aortic (DAo) blood velocities from stage 24 embryos with an ultrasound pulsed-Doppler flow meter; and the left and right third and fourth aortic arch blood flow with a laser-Doppler flow meter. Ventricular and atrial cross-sectional areas were measured from sequential video fields for planimetry. Intracardiac flow patterns were imaged on video by injecting India ink into the vitelline vein. In separate embryos, radiopaque microfil was injected into the cardiovascular system for micro-CT scanning. We analyzed the morphologic characteristics of the heart at stage 34. Active AV and DAo stroke volume (mm(3)), right third and fourth aortic arch blood flow (mm(3)/s) were all decreased in ligated embryos (P < 0.05) when compared with normal and sham embryos. Ventricular end-diastolic volume versus normal and sham embryos decreased by 45% and 46%, respectively (P < 0.05). India ink injection revealed altered right aortic arch flow patterns in the ligated embryos compared with normal embryos. micro-CT imaging confirmed altered arch morphogenesis. Alterations in intracardiac blood flow disrupt both early cardiac morphogenesis and aortic arch selection. |
تدمد: | 1932-8494 1932-8486 |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a6d351164afb2ec35798c9e0a820f262 https://doi.org/10.1002/ar.20885 |
حقوق: | CLOSED |
رقم الأكسشن: | edsair.doi.dedup.....a6d351164afb2ec35798c9e0a820f262 |
قاعدة البيانات: | OpenAIRE |
تدمد: | 19328494 19328486 |
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