A strategy to replace the mouse bioassay for detecting and identifying lipophilic marine biotoxins by combining the neuro-2a bioassay and LC-MS/MS analysis
| العنوان: | A strategy to replace the mouse bioassay for detecting and identifying lipophilic marine biotoxins by combining the neuro-2a bioassay and LC-MS/MS analysis |
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| المؤلفون: | Toine F H Bovee, Liza Portier, Peter J M Hendriksen, Ron L.A.P. Hoogenboom, Mirjam D. Klijnstra, Arjen Gerssen, Marcia Bodero, Leonardo Guzmán |
| المصدر: | Marine Drugs, 16(12) Marine Drugs Volume 16 Issue 12 Marine Drugs, Vol 16, Iss 12, p 501 (2018) Marine Drugs 16 (2018) 12 |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Novel Foods & Agrochains, Pharmaceutical Science, Novel Foods & Agroketens, 01 natural sciences, Mice, BU Contaminants & Toxins, Mouse bioassay, Tandem Mass Spectrometry, Drug Discovery, False positive paradox, Bioassay, BU Toxicology, Novel Foods & Agrochains, lcsh:QH301-705.5, Pharmacology, Toxicology and Pharmaceutics (miscellaneous), Chromatography, High Pressure Liquid, Oxocins, BU Toxicology, BU Toxicologie, Novel Foods & Agroketens, Ethical concerns, Fish |
| الوصف: | Marine biotoxins in fish and shellfish can cause several symptoms in consumers, such as diarrhea, amnesia, or even death by paralysis. Monitoring programs are in place for testing shellfish on a regular basis. In some countries testing is performed using the so-called mouse bioassay, an assay that faces ethical concerns not only because of animal distress, but also because it lacks specificity and results in high amounts of false positives. In Europe, for lipophilic marine biotoxins (LMBs), a chemical analytical method using LC-MS/MS was developed as an alternative and is now the reference method. However, safety is often questioned when relying solely on such a method, and as a result, the mouse bioassay might still be used. In this study the use of a cell-based assay for screening, i.e., the neuro-2a assay, in combination with the official LC-MS/MS method was investigated as a new alternative strategy for the detection and quantification of LMBs. To this end, samples that had been tested previously with the mouse bioassay were analyzed in the neuro-2a bioassay and the LC-MS/MS method. The neuro-2a bioassay was able to detect all LMBs at the regulatory levels and all samples that tested positive in the mouse bioassay were also suspect in the neuro-2a bioassay. In most cases, these samples contained toxin levels (yessotoxins) that explain the outcome of the bioassay but did not exceed the established maximum permitted levels. |
| وصف الملف: | application/octet-stream; application/pdf |
| اللغة: | English |
| تدمد: | 1660-3397 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a6de98ea6e676b14b0180e5ccbf9a458 https://research.wur.nl/en/publications/a-strategy-to-replace-the-mouse-bioassay-for-detecting-and-identi |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....a6de98ea6e676b14b0180e5ccbf9a458 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16603397 |
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