Effect of mitochondrial viability and metabolism on technetium-99m-sestamibi myocardial retention
| العنوان: | Effect of mitochondrial viability and metabolism on technetium-99m-sestamibi myocardial retention |
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| المؤلفون: | Cesare Orlandi, Paul D. Crane, Robert J. Laliberté, Martin J.M.C. Thoolen, Stuart J. Heminway |
| المصدر: | European Journal of Nuclear Medicine. 20:20-25 |
| بيانات النشر: | Springer Science and Business Media LLC, 1993. |
| سنة النشر: | 1993 |
| مصطلحات موضوعية: | Male, Technetium Tc 99m Sestamibi, Ruthenium red, Cell Survival, medicine.drug_class, Guinea Pigs, Myocardial Ischemia, Ischemia, chemistry.chemical_element, Myocardial Reperfusion Injury, Calcium channel blocker, Mitochondrion, Calcium, Pharmacology, Mitochondria, Heart, chemistry.chemical_compound, Respiration, medicine, Animals, Radiology, Nuclear Medicine and imaging, Radionuclide Imaging, Heart metabolism, Chemistry, Heart, General Medicine, Anatomy, medicine.disease, Ruthenium Red |
| الوصف: | This study investigated the mechanism of myocardial retention of technetium-99m-sestamibi. 99mTc-sestamibi was injected intravenously into guinea pigs, and the myocardium was homogenized and fractionated by differential centrifugation. More than 90% of myocardial 99mTc-sestamibi was localized within the mitochondrial fraction. Calcium was found to release 99mTc-sestamibi from the mitochondrial fraction, with an IC50 of 2.54 +/- 0.98 mM. This effect was potentiated by NaCl, and inhibited by the mitochondrial calcium channel blocker ruthenium red. In vitro uptake of 99mTc-sestamibi was found to increase from 10.5% +/- 3.0% to 61.2% +/- 0.2% with the addition of 10 mM succinate, indicating that respiration is involved. Since irreversible ischemia results in cellular and mitochondrial calcium "overload" and loss of mitochondrial metabolic function, 99mTc-sestamibi should not be retained in necrotic or irreversibly ischemic myocardium, and could potentially act as a sensitive indicator of myocardial cell viability. |
| تدمد: | 1619-7089 0340-6997 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a6edc7ae764699de84511ef05325401f https://doi.org/10.1007/bf02261241 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....a6edc7ae764699de84511ef05325401f |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 16197089 03406997 |
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