Improving probes for super-resolution
| العنوان: | Improving probes for super-resolution |
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| المؤلفون: | Wesley R. Legant, Regan P. Moore |
| المصدر: | Nature methods |
| بيانات النشر: | Springer Science and Business Media LLC, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Materials science, business.industry, Oligonucleotides, DNA, Cell Biology, Biochemistry, Superresolution, Article, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, Optics, Microscopy, Fluorescence, business, Molecular Biology, 030217 neurology & neurosurgery, Biotechnology |
| الوصف: | Super-resolution methods allow the visualization of phenomena thus far elusive to light microscopy. While current implementations are technically approaching true molecular-scale resolution, this does not translate to imaging biological specimen, due to the unavailability of small affinity reagents. Here, we introduce SOMAmers as labeling reagents for DNA-PAINT. We demonstrate the achievable resolution and specificity by labeling and imaging of transmembrane as well as intracellular targets in fixed and live cell-specimen. |
| تدمد: | 1548-7105 1548-7091 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a718b10d61df7b6b956ac128e5960644 https://doi.org/10.1038/s41592-018-0120-1 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....a718b10d61df7b6b956ac128e5960644 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 15487105 15487091 |
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