The Important Role of Dendritic Cell (DC) in iNKT-Mediated Modulation of NK Cell Function in Chlamydia pneumoniae Lung Infection
| العنوان: | The Important Role of Dendritic Cell (DC) in iNKT-Mediated Modulation of NK Cell Function in Chlamydia pneumoniae Lung Infection |
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| المؤلفون: | Antony George Joyee, Shuhe Wang, Jie Yang, Xi Yang, Weiming Zhao, Hong Bai, Xiaoling Gao, Lei Zhao |
| المصدر: | Mediators of Inflammation Mediators of Inflammation, Vol 2019 (2019) |
| بيانات النشر: | Hindawi Limited, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Lung Diseases, 0301 basic medicine, Adoptive cell transfer, Article Subject, Immunology, Cell, Biology, Interferon-gamma, Mice, 03 medical and health sciences, 0302 clinical medicine, In vivo, lcsh:Pathology, medicine, Animals, Chlamydophila Infections, Mice, Knockout, Chlamydia, Dendritic Cells, Cell Biology, Dendritic cell, Chlamydophila pneumoniae, medicine.disease, Cell function, In vitro, Tumor Necrosis Factor Receptor Superfamily, Member 7, 3. Good health, Killer Cells, Natural, Mice, Inbred C57BL, 030104 developmental biology, medicine.anatomical_structure, Natural Killer T-Cells, Female, Function (biology), lcsh:RB1-214, Research Article, 030215 immunology |
| الوصف: | Chlamydia pneumoniae(Cpn) infection causes multiple acute and chronic human diseases. The role of DCs in host defense against Cpn infection has been well documented. The same is true for invariant natural killer T (iNKT) cells and NK cells, but the interaction among cells is largely unknown. In this study, we investigated the influence and mechanism of iNKT cell on the differentiation and function of NK cell inCpnlung infection and the role played by DCs in this process. We found that expansion of IFN-γ-producing NK cells quickly happened after the infection, but this response was altered in iNKT knockout (KO) mice. The expression of activation markers and the production of IFN-γby different NK subsets were significantly lower in KO mice than wild-type (WT) mice. Using in vitro DC-NK coculture and in vivo adoptive transfer approaches, we further examined the role of DCs in iNKT-mediated modulation of NK cell function. We found that NK cells expressed lower levels of activation markers and produced less IFN-γwhen they were cocultured with DCs from KO mice than WT mice. More importantly, we found that the adoptive transfer of DCs from the KO mice induced less NK cell activation and IFN-γproduction. The results provided evidence on the modulating effect of iNKT cell on NK cell function, particularly the critical role of DCs in this modulation process. The finding suggests the complexity of cellular interactions inCpnlung infection, which should be considered in designing preventive and therapeutic approaches for diseases and infections. |
| وصف الملف: | text/xhtml |
| تدمد: | 1466-1861 0962-9351 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a72b1870181de21c76d9746d8120b3d6 https://doi.org/10.1155/2019/4742634 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....a72b1870181de21c76d9746d8120b3d6 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14661861 09629351 |
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