Overcoming the blood–brain barrier by Annexin A1-binding peptide to target brain tumours
| العنوان: | Overcoming the blood–brain barrier by Annexin A1-binding peptide to target brain tumours |
|---|---|
| المؤلفون: | Chun-Teng Huang, Minoru Fukuda, Yoichi Suwa, Toshio Sasai, Motohiro Nonaka, Michiko N. Fukuda, Kazuhiro Sugihara, Tomoya O. Akama, Kazuhiko Yamasaki, Jun Nakayama, Itsuko Kimura-Takagi, Takashi Yaegashi, Jiunn Chern Yeh, Hideaki Mabashi-Asazuma, Chizuko Nishizawa-Harada, Alexandre Rosa Campos, Misa Suzuki-Anekoji, Toshiaki K. Shibata, Masato Nagaoka, Donald L. Jarvis |
| المصدر: | British Journal of Cancer |
| بيانات النشر: | Springer Science and Business Media LLC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Cancer Research, Cancer therapy, Antineoplastic Agents, Mice, SCID, Scid mice, Blood–brain barrier, Solutol-HS15, Article, Mice, 03 medical and health sciences, 0302 clinical medicine, Immune system, medicine, Animals, Humans, Annexin A1, 030304 developmental biology, Drug Carriers, 0303 health sciences, Brain Neoplasms, Chemistry, Melanoma, medicine.disease, Binding peptide, Rats, Mice, Inbred C57BL, CNS cancer, medicine.anatomical_structure, Oncology, Blood-Brain Barrier, Cell culture, 030220 oncology & carcinogenesis, Cancer research, Peptides |
| الوصف: | Background Annexin A1 is expressed specifically on the tumour vasculature surface. Intravenously injected IF7 targets tumour vasculature via annexin A1. We tested the hypothesis that IF7 overcomes the blood–brain barrier and that the intravenously injected IF7C(RR)-SN38 eradicates brain tumours in the mouse. Methods (1) A dual-tumour model was generated by inoculating luciferase-expressing melanoma B16 cell line, B16-Luc, into the brain and under the skin of syngeneic C57BL/6 mice. IF7C(RR)-SN38 was injected intravenously daily at 7.0 μmoles/kg and growth of tumours was assessed by chemiluminescence using an IVIS imager. A similar dual-tumour model was generated with the C6-Luc line in immunocompromised SCID mice. (2) IF7C(RR)-SN38 formulated with 10% Solutol HS15 was injected intravenously daily at 2.5 μmoles/kg into two brain tumour mouse models: B16-Luc cells in C57BL/6 mice, and C6-Luc cells in nude mice. Results (1) Daily IF7C(RR)-SN38 injection suppressed tumour growth regardless of cell lines or mouse strains. (2) Daily injection of Solutol-formulated IF7C(RR)-SN38 led into complete disappearance of B16-Luc brain tumour in C57BL/6 mice, whereas this did not occur in C6-Luc in nude mice. Conclusions IF7C(RR)-SN38 crosses the blood–brain barrier and suppresses growth of brain tumours in mouse models. Solutol HS15-formulated IF7C(RR)-SN38 may have promoted an antitumour immune response. |
| تدمد: | 1532-1827 0007-0920 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a74615f6b64e73fceabf514e8e359b9f https://doi.org/10.1038/s41416-020-01066-2 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....a74615f6b64e73fceabf514e8e359b9f |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 15321827 00070920 |
|---|