Animal model of intrahepatic metastasis of hepatocellular carcinoma: establishment and characteristic
| العنوان: | Animal model of intrahepatic metastasis of hepatocellular carcinoma: establishment and characteristic |
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| المؤلفون: | Jike Hu, Maswikiti Ewetse Paul, Zedong Feng, Xueyan Wang, Xuemei Li, Baohong Gu, Yanling Ma, Hao Chen, Yang Yu, Bofang Wang |
| المصدر: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-6 (2020) |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Carcinoma, Hepatocellular, lcsh:Medicine, Spleen, Mice, SCID, Article, Metastasis, 03 medical and health sciences, Mice, 0302 clinical medicine, In vivo, Cell Line, Tumor, medicine, Carcinoma, Bioluminescence imaging, Intrahepatic metastasis, Animals, Humans, Neoplasm Metastasis, lcsh:Science, Cancer models, Multidisciplinary, business.industry, lcsh:R, Liver Neoplasms, medicine.disease, Xenograft Model Antitumor Assays, digestive system diseases, Disease Models, Animal, 030104 developmental biology, medicine.anatomical_structure, Liver, Cell culture, Hepatocellular carcinoma, Cancer research, lcsh:Q, 030211 gastroenterology & hepatology, business, Neoplasm Transplantation |
| الوصف: | One of the most important and striking characteristics of hepatocellular carcinoma (HCC) with intrahepatic metastasis, is that it results in extremely poor prognosis. Animal models have become a fundamental and very useful in research for disease study. However, some limitation has arisen from these model systems. We have therefore established a model of HCC with intrahepatic metastasis and noticed some differential appearances in different HCC cell lines. Luciferase-transfected HCC cell lines MHCC97-H and PLC/PRF/5 were inoculated into SCID mice via spleen. Observation the intrahepatic metastasis by bioluminescence imaging in vivo and comparing of the differential formation of metastatic lesions between different HCC cell lines by incorporating physical anatomy was done. Animal models for HCC intrahepatic metastasis were well established. However, there were some clearly noticed differences between MHCC97-H and PLC/PRF/5 cell lines. The group of MHCC97-H cell line readily metastasis in the liver, whereas group PLC/PRF/5 cell line developed extensive intrahepatic metastasis and formed large tumor in situ in the spleen. MHCC97-H and PLC/PRF/5 cell lines can be used to successfully establish a model of HCC intrahepatic metastasis with distinctive characteristics, which provides an important direction for the study of the mechanism of HCC intrahepatic metastasis, and may hopefully provide a basis for clinical treatment. |
| تدمد: | 2045-2322 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a74e154703e34abc87af68bae70b157b https://pubmed.ncbi.nlm.nih.gov/32939004 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....a74e154703e34abc87af68bae70b157b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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