Bioavailable wine pomace attenuates oxalate-induced type II epithelial mesenchymal transition and preserve the differentiated phenotype of renal MDCK cells
| العنوان: | Bioavailable wine pomace attenuates oxalate-induced type II epithelial mesenchymal transition and preserve the differentiated phenotype of renal MDCK cells |
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| المؤلفون: | María C. Fernández Tome, Gisela Gerardi, Mónica Cavia-Saiz, Cecilia Perazzo, María D. Rivero-Pérez, María Luisa González-Sanjosé, Pilar Muñiz, Cecilia I. Casali |
| المصدر: | Heliyon, Vol 6, Iss 11, Pp e05396-(2020) Repositorio Institucional de la Universidad de Burgos (RIUBU) instname Heliyon |
| بيانات النشر: | Elsevier, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Polyphenol, Bioquímica, 0301 basic medicine, Cell biology, Molecular biology, Cellular differentiation, Cell, Inflammation, Cancer research, Biochemistry, Cell junction, Oxalate, Natural product, Food science, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Alimentos, medicine, Cell differentiation, Epithelial–mesenchymal transition, lcsh:Social sciences (General), lcsh:Science (General), Cytoskeleton, Plant products, Renal system, Wine pomace, Kidney, Biología molecular, Multidisciplinary, Cadherin, Chemistry, EMT, Polyphenols, E-cadherin, food and beverages, 030104 developmental biology, medicine.anatomical_structure, Food, lcsh:H1-99, Antioxidant, medicine.symptom, 030217 neurology & neurosurgery, Research Article, lcsh:Q1-390 |
| الوصف: | The functional renal epithelium is composed of differentiated and polarized tubular cells with a strong actin cortex and specialized cell-cell junctions. If, under pathological conditions, these cells have to resist higher kidney osmolarity, they need to activate diverse mechanisms to survive external nephrotoxic agents such as inflammation and oxidative stress. Wine pomace polyphenols exert protective effects on renal cells. In this study, two wine-pomace products and their protective effects upon promotion and preservation of normal cell differentiation and attenuation of oxalate-induced type II epithelial mesenchymal transition (EMT) are evaluated. Treatment with gastrointestinal and colonic bioavailable fractions from red (rWPP) and white (wWPP) wine pomaces, both in the presence and the absence of oxalate, showed similar cell numbers and nuclear size than the non-treated differentiated MDCK cells. Immunofluorescence analysis showed the reduction of morphological changes and the preservation of cellular junctions for the rWPP and wWPP pre-treatment of cells exposed to oxalate injury. Hence, both rWPP and wWPP attenuated oxalate type II EMT in MDCK cells that conserved their epithelial morphology and cellular junctions through the antioxidant activities of grape pomace polyphenols. Food Science; Cell Biology; Plant Products; Cell Differentiation; Cytoskeleton; Natural product; Polyphenol; Antioxidant; Cancer Research; Renal System; wine pomace; polyphenols; EMT; oxalate; E-cadherin |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2405-8440 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a7805ee7e144c9b89ee2455ea92db803 http://www.sciencedirect.com/science/article/pii/S2405844020322398 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....a7805ee7e144c9b89ee2455ea92db803 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 24058440 |
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