H2A.B is a cancer/testis factor involved in the activation of ribosome biogenesis in Hodgkin lymphoma
العنوان: | H2A.B is a cancer/testis factor involved in the activation of ribosome biogenesis in Hodgkin lymphoma |
---|---|
المؤلفون: | Jiayu Wen, Elizabeth Paver, Tatiana A. Soboleva, Amanda Bullman, Xuanzhao Jiang, David J. Tremethick, Yu-Huan Wu, Gege Sun, Jane E. Dahlstrom |
المصدر: | EMBO Rep |
بيانات النشر: | EMBO, 2021. |
سنة النشر: | 2021 |
مصطلحات موضوعية: | Male, biology, Nucleolus, RNA, Ribosome biogenesis, RNA polymerase II, Articles, Hodgkin Disease, Biochemistry, Chromatin, Cell biology, Histones, Transcription (biology), Testis, Gene expression, Genetics, biology.protein, RNA polymerase I, Humans, Ribosomes, Molecular Biology |
الوصف: | Testis-specific regulators of chromatin function are commonly ectopically expressed in human cancers, but their roles are poorly understood. Examination of 81 primary Hodgkin lymphoma (HL) samples showed that the ectopic expression of the eutherian testis-specific histone variant H2A.B is an inherent feature of HL. In experiments using two HL cell lines derived from different subtypes of HL, H2A.B knockdown inhibited cell proliferation. H2A.B was enriched in both nucleoli of these HL cell lines and primary HL samples. We found that H2A.B enhanced ribosomal DNA (rDNA) transcription, was enriched at the rDNA promoter and transcribed regions, and interacted with RNA Pol I. Depletion of H2A.B caused the loss of RNA Pol I from rDNA chromatin. Remarkably, H2A.B was also required for high levels of ribosomal protein gene expression being located at the transcriptional start site and within the gene body. H2A.B knockdown reduced gene body chromatin accessibility of active RNA Pol II genes concurrent with a decrease in transcription. Taken together, our data show that in HL H2A.B has acquired a new function, the ability to increase ribosome biogenesis. |
تدمد: | 1469-3178 1469-221X |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a79bf894a719795b9133861ce4ffa5b3 https://doi.org/10.15252/embr.202152462 |
حقوق: | OPEN |
رقم الأكسشن: | edsair.doi.dedup.....a79bf894a719795b9133861ce4ffa5b3 |
قاعدة البيانات: | OpenAIRE |
تدمد: | 14693178 1469221X |
---|