Gsx2 is required for specification of neurons in the inferior olivary nuclei from Ptf1a-expressing neural progenitors in zebrafish
| العنوان: | Gsx2 is required for specification of neurons in the inferior olivary nuclei from Ptf1a-expressing neural progenitors in zebrafish |
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| المؤلفون: | Marina Sakagami, Tsubasa Itoh, Takashi Shimizu, Miki Takeuchi, Koichi Kawakami, Masahiko Hibi, Kenta Sumiyama, Kazuhide Asakawa |
| المصدر: | Development. |
| بيانات النشر: | The Company of Biologists, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Cerebellum, Neurogenesis, Mutant, Retinoic acid, Fibroblast growth factor, medicine.disease_cause, chemistry.chemical_compound, Neural Stem Cells, medicine, Animals, Hox gene, Molecular Biology, Zebrafish, Homeodomain Proteins, Neurons, Mutation, biology, Gene Expression Regulation, Developmental, Zebrafish Proteins, biology.organism_classification, Cell biology, medicine.anatomical_structure, chemistry, Homeobox, Transcription Factors, Developmental Biology |
| الوصف: | Neurons in the inferior olivary nuclei (IO neurons) send climbing fibers to Purkinje cells to elicit functions of the cerebellum. IO neurons and Purkinje cells are derived from neural progenitors expressing the proneural gene ptf1a. In this study, we found that the homeobox gene gsx2 was co-expressed with ptf1a in IO progenitors in zebrafish. Both gsx2 and ptf1a zebrafish mutants showed a strong reduction or loss of IO neurons. The expression of ptf1a was not affected in gsx2 mutants and vice versa. In IO progenitors, the ptf1a mutation increased apoptosis whereas the gsx2 mutation did not, suggesting that ptf1a and gsx2 are regulated independently of each other and have distinct roles. The fibroblast growth factors (Fgf) 3/8a and retinoic acid signals negatively and positively, respectively, regulated gsx2 expression and thereby the development of IO neurons. mafba and hox genes are at least partly involved in the Fgf- and retinoic acid-dependent regulation of IO neuronal development. Our results indicate that gsx2 mediates the rostro-caudal positional signals to specify the identity of IO neurons from ptf1a-expressing neural progenitors. |
| تدمد: | 1477-9129 0950-1991 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a80e091f2df53b7634d0e83559fee7b5 https://doi.org/10.1242/dev.190603 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....a80e091f2df53b7634d0e83559fee7b5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14779129 09501991 |
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