Coinfection by Severe Acute Respiratory Syndrome Coronavirus 2 and Influenza A(H1N1)pdm09 Virus Enhances the Severity of Pneumonia in Golden Syrian Hamsters
| العنوان: | Coinfection by Severe Acute Respiratory Syndrome Coronavirus 2 and Influenza A(H1N1)pdm09 Virus Enhances the Severity of Pneumonia in Golden Syrian Hamsters |
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| المؤلفون: | Anna Jinxia Zhang, Vincent Kwok-Man Poon, Kelvin K. W. To, Shuofeng Yuan, Pui Wang, Hin Chu, Chris Chung-Sing Chan, Honglin Chen, Yanxia Chen, Andrew Chak-Yiu Lee, Kwok-Yung Yuen, Fei-Fei Liu, Siu Ying Lau, Jasper Fuk-Woo Chan, Can Li |
| المصدر: | Clinical Infectious Diseases Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America |
| بيانات النشر: | Oxford University Press (OUP), 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Microbiology (medical), viruses, 030106 microbiology, Influenza B, medicine.disease_cause, Virus, 03 medical and health sciences, Mice, Virus antigen, Influenza A Virus, H1N1 Subtype, Cricetinae, respiratory distress, Influenza, Human, medicine, Animals, Humans, skin and connective tissue diseases, Neutralizing antibody, influenza A, Coronavirus, biology, Mesocricetus, business.industry, Coinfection, SARS-CoV-2, virus diseases, COVID-19, medicine.disease, Virology, respiratory tract diseases, Pneumonia, Titer, Disease Models, Animal, Editorial Commentary, 030104 developmental biology, AcademicSubjects/MED00290, Infectious Diseases, biology.protein, business, Viral load |
| الوصف: | Background Clinical outcomes of the interaction between the co-circulating pandemic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and seasonal influenza viruses are unknown. Methods We established a golden Syrian hamster model coinfected by SARS-CoV-2 and mouse-adapted A(H1N1)pdm09 simultaneously or sequentially. The weight loss, clinical scores, histopathological changes, viral load and titer, and serum neutralizing antibody titer were compared with hamsters challenged by either virus. Results Coinfected hamsters had more weight loss, more severe lung inflammatory damage, and tissue cytokine/chemokine expression. Lung viral load, infectious virus titers, and virus antigen expression suggested that hamsters were generally more susceptible to SARS-CoV-2 than to A(H1N1)pdm09. Sequential coinfection with A(H1N1)pdm09 one day prior to SARS-CoV-2 exposure resulted in a lower lung SARS-CoV-2 titer and viral load than with SARS-CoV-2 monoinfection, but a higher lung A(H1N1)pdm09 viral load. Coinfection also increased intestinal inflammation with more SARS-CoV-2 nucleoprotein expression in enterocytes. Simultaneous coinfection was associated with delay in resolution of lung damage, lower serum SARS-CoV-2 neutralizing antibody, and longer SARS-CoV-2 shedding in oral swabs compared to that of SARS-CoV-2 monoinfection. Conclusions Simultaneous or sequential coinfection by SARS-CoV-2 and A(H1N1)pdm09 caused more severe disease than monoinfection by either virus in hamsters. Prior A(H1N1)pdm09 infection lowered SARS-CoV-2 pulmonary viral loads but enhanced lung damage. Whole-population influenza vaccination for prevention of coinfection, and multiplex molecular diagnostics for both viruses to achieve early initiation of antiviral treatment for improvement of clinical outcome should be considered. |
| اللغة: | English |
| تدمد: | 1537-6591 1058-4838 |
| DOI: | 10.1093/cid/ciaa1747 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a86e8cbf40ea8a6716196b1e7c4914b3 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....a86e8cbf40ea8a6716196b1e7c4914b3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15376591 10584838 |
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| DOI: | 10.1093/cid/ciaa1747 |