The Novel microRNA Rno-miR-686-3p Is Associated with the Ischaemic Penumbra
| العنوان: | The Novel microRNA Rno-miR-686-3p Is Associated with the Ischaemic Penumbra |
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| المؤلفون: | Wei, Wang, Jin-Pin, Li, Jing-Li, Liu |
| المصدر: | European Neurology. 85:224-234 |
| بيانات النشر: | S. Karger AG, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Stroke, MicroRNAs, Neurology, Animals, Infarction, Middle Cerebral Artery, Neurology (clinical), Biomarkers, Brain Ischemia, Rats |
| الوصف: | Introduction: We explored microRNA (miRNA) profiles correlated with the penumbra in three different phases of ischaemic stroke, using a permanent middle cerebral artery occlusion (p-MCAO) rat model. Materials and Methods: A 2-mm coronal section was cut from the optic chiasma in the caudal direction, and the penumbra was located in the area between a longitudinal line approximately 2 mm from the midline and a transverse diagonal line at the “2-o’clock” position. Total RNA was extracted from tissue specimens and peripheral blood samples, followed by deep sequencing analysis. Results: We identified nine novel miRNA candidates in tissues and evaluated their expression levels using real-time quantitative polymerase chain reaction. In situ hybridization was conducted to assess miRNA localization in the brain. Of these nine candidates, we identified and characterized a novel miRNA, rno-miR-686-3p, which was localized in cell nuclei of the cortex, and associated with the penumbra. rno-miR-686-3p was downregulated at 1 (p = 0.042), 3 (p = 0.032), and 4 h (p = 0.007) post-p-MCAO in the penumbra. A total of 297 potential target genes were predicted. Moreover, functional annotation clustering and pathway enrichment analysis predicted that rno-miR-686-3p participates in transcriptional regulation and the Wnt and cyclic adenosine monophosphate (cAMP) signalling pathways. Conclusion: rno-miR-686-3p is a novel miRNA associated with the ischaemic penumbra that is implicated in transcriptional regulation and modulation of the Wnt and cAMP signalling pathways. Furthermore, it may serve as a possible new biomarker with potential value for detecting the existence of the penumbra. |
| تدمد: | 1421-9913 0014-3022 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a8828a179d49dbbe34fac07100ce371c https://doi.org/10.1159/000521491 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....a8828a179d49dbbe34fac07100ce371c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14219913 00143022 |
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