Neuropeptide B promotes proliferation and differentiation of rat brown primary preadipocytes
| العنوان: | Neuropeptide B promotes proliferation and differentiation of rat brown primary preadipocytes |
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| المؤلفون: | Priyavathi Dhandapani, Maria Billert, Marek Skrzypski, Mathias Z. Strowski, Krzysztof W. Nowak, Dawid Szczepankiewicz, Tatiana Wojciechowicz, Oskar Wasielewski |
| المصدر: | FEBS Open Bio FEBS Open Bio, Vol 11, Iss 4, Pp 1153-1164 (2021) |
| بيانات النشر: | Wiley, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | preadipocytes, 0301 basic medicine, Neuropeptide B, Cell Survival, proliferation, p38 mitogen-activated protein kinases, Models, Biological, General Biochemistry, Genetics and Molecular Biology, adipogenesis, 03 medical and health sciences, 0302 clinical medicine, Adipose Tissue, Brown, Brown adipose tissue, medicine, Animals, neuropeptide B, Receptor, lcsh:QH301-705.5, Cells, Cultured, Research Articles, Cell Proliferation, PRDM16, Messenger RNA, Chemistry, Stem Cells, Neuropeptides, Cell Differentiation, brown adipose tissue, differentiation, Rats, Cell biology, Adipocytes, Brown, 030104 developmental biology, medicine.anatomical_structure, lcsh:Biology (General), Adipogenesis, 030220 oncology & carcinogenesis, Intracellular, Research Article |
| الوصف: | Neuropeptide B (NPB) is reported to regulate energy homeostasis and metabolism via the NPBWR1 and NPBWR2 receptors in various tissues. However, the molecular mechanisms triggered from their interaction are not well investigated in brown adipose tissue. In this study, we specifically analyzed the role of NPB in controlling brown adipogenesis in rat brown preadipocytes. We first detected the expression of NPBWR1 and NPB on mRNA and protein level in brown preadipocytes and observed that NPB increased viability and proliferation of preadipocytes. Moreover, NPB stimulated expression of adipogenic genes (Prdm16, Ucp1) and suppressed the expression of antiadipogenic preadipocyte factor 1 (Pref1) during the differentiation process. Altogether, this led to an increase in intracellular lipid accumulation during preadipocyte differentiation, coupled with an increase in adrenaline‐induced oxygen consumption mediated by NPB. Furthermore, Ucp1 expression stimulated by NPB was attenuated by blockade of p38 kinase. In summary, we conclude that NPB promotes proliferation and differentiation of rat brown preadipocytes via p38‐dependent mechanism and plays an important role in controlling brown adipose tissue formation. Neuropeptide B (NPB) and NPBWR1 are expressed in brown preadipocytes. NPB increased viability and proliferation of preadipocytes. NPB stimulated expression of adipogenic genes (Prdm16, Ucp1) and lipid accumulation. Ucp1 expression stimulated by NPB was attenuated by blockade of p38 kinase. We conclude that NPB modulates brown adipogenesis via p38‐dependent mechanism. |
| تدمد: | 2211-5463 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a8c54e1135690473dec89aa99e6caa29 https://doi.org/10.1002/2211-5463.13128 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....a8c54e1135690473dec89aa99e6caa29 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22115463 |
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