Isolation and Structural Determination of Two Novel Phlorotannins from the Brown Alga Ecklonia kurome Okamura, and Their Radical Scavenging Activities
| العنوان: | Isolation and Structural Determination of Two Novel Phlorotannins from the Brown Alga Ecklonia kurome Okamura, and Their Radical Scavenging Activities |
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| المؤلفون: | Yi Chin Lin, Takafumi Uchida, Shinya Segawa, Keiichi Konoki, Mari Yotsu-Yamashita, Yuko Cho, Hisatomi Ito, Sawako Kondo, Haruhiko Toyohara |
| المصدر: | Marine Drugs; Volume 11; Issue 1; Pages: 165-183 Marine Drugs, Vol 11, Iss 1, Pp 165-183 (2013) Marine Drugs |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Ecklonia kurome, Antioxidant, DPPH, medicine.medical_treatment, Phloroglucinol, alpha-Tocopherol, phlorotannins, Pharmaceutical Science, Ascorbic Acid, Dioxins, Phaeophyta, radical scavenging activity, Antioxidants, Mass Spectrometry, Article, Cell Line, chemistry.chemical_compound, Mice, Picrates, structural determination, Cell Line, Tumor, Drug Discovery, medicine, Organic chemistry, Animals, Humans, Pharmacology, Toxicology and Pharmaceutics (miscellaneous), lcsh:QH301-705.5, Benzofurans, biology, Biphenyl Compounds, Polyphenols, Free Radical Scavengers, biology.organism_classification, Ascorbic acid, Fluoresceins, NMR, Dieckol, Biphenyl compound, chemistry, lcsh:Biology (General), Polyphenol, Tannins |
| الوصف: | Two novel phlorotannins with a molecular weight of 974, temporarily named 974-A and 974-B, were isolated from the polyphenol powder prepared from the edible marine brown alga Ecklonia kurome Okamura, and their chemical structures were determined by spectroscopic method. The isolated yield of the total of 974-A and 974-B was approximately 4% (w/w) from the polyphenol powder. In 974-A, the carbon at the C2′ position in the A ring of phlorofucofuroeckol-A forms a C–C bond with the carbon at the C2″ position of the C ring of triphloretol-B, while in 974-B, phlorofucofuroeckol-B and triphloretol-B form a C–C bond in the same manner as in 974-A. These structures were supported by high resolution-MS/MS data. To evaluate the antioxidant activities, the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and intracellular radical scavenging assay, using 2′,7′-dichlorofluorescin diacetate (DCFH-DA), were performed for 974-A, 974-B, and four known phlorotannins. The results of the DPPH assay showed that the IC50 values of 974-A, 974-B, phlorofucofuroeckol-A, and dieckol were significantly smaller than those of phlorofucofuroeckol-B, phloroglucinol, α-tocopherol, and ascorbic acid. Furthermore, the DCFH-DA assay suggested that 974-A, 974-B, and dieckol reduce intracellular reactive oxygen species most strongly among the tested compounds. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1660-3397 |
| DOI: | 10.3390/md11010165 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a99ad6f0c4d9550702334378663303b2 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....a99ad6f0c4d9550702334378663303b2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16603397 |
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| DOI: | 10.3390/md11010165 |