Molecular characterization of Dobrava-Belgrade hantavirus in Serbia, 2007–2011
| العنوان: | Molecular characterization of Dobrava-Belgrade hantavirus in Serbia, 2007–2011 |
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| المؤلفون: | Maja Stanojevic, Gorana Stamenković, Valentina Cirkovic, Ana Gligic, Marina Siljic |
| المصدر: | Journal of Infection and Public Health, Vol 12, Iss 5, Pp 645-649 (2019) Journal of Infection and Public Health |
| بيانات النشر: | Elsevier, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, medicine.medical_specialty, Orthohantavirus, Genotype, Hantavirus Infections, viruses, 030106 microbiology, Biology, Antibodies, Viral, Virus, Serology, lcsh:Infectious and parasitic diseases, 03 medical and health sciences, 0302 clinical medicine, Blood serum, Phylogenetics, Molecular genetics, medicine, Humans, lcsh:RC109-216, 030212 general & internal medicine, Phylogeny, Hantavirus, Phylogenetic tree, lcsh:Public aspects of medicine, Public Health, Environmental and Occupational Health, Genetic Variation, Molecular, virus diseases, lcsh:RA1-1270, General Medicine, Sequence Analysis, DNA, Amplicon, Virology, 3. Good health, Dobrava-Belgrade hantavirus, Infectious Diseases, Hemorrhagic Fever with Renal Syndrome, RNA, Viral, Serbia |
| الوصف: | Background: Hantaviruses are etiological agents of emerging zoonotic diseases worldwide, including hemorrhagic fever with renal syndrome (HFRS). A number of hantavirus species is known to be present in Europe. In Serbia, existing data on hantavirus presence and prevalence rely in serological findings. In this study, molecular analysis was performed in order to characterize HFRS causing hantaviruses in Serbia. Methods: Sixty four serum samples of HFRS cases, previously found seropositive to anti-hantaviral anti-bodies, were included in the study. Partial hantaviral L and S segments were PCR amplified producing 390nt and 598nt amplicons, respectively, in parallel with human beta-actin mRNA as external reverse transcription positive control. Hantavirus specific PCR products were DNA sequenced in both direction and the obtained sequences phylogenetically confirmed and analyzed. Results: PCR detection of hantavirus L and S genome segments was positive in 18/64 and 11/64 tested samples, respectively. Positive PCR results involved samples obtained from different locations, mostly from central and southern parts of Serbia. All the obtained sequences were identified as Dobrava-Belgrade virus (DOBV). In the phylogenetic analysis sequences from Serbia tended to cluster in distinctive, geographically related clusters. Conclusions: Our findings indicate DOBV as the main HFRS causing hantavirus in Serbia, the site of its initial isolation. (C) 2019 Published by Elsevier Limited on behalf of King Saud Bin Abdulaziz University for Health Sciences. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
| اللغة: | English |
| تدمد: | 1876-0341 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a9a52da119e3a1671efb8e35652f72ea http://www.sciencedirect.com/science/article/pii/S187603411930108X |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....a9a52da119e3a1671efb8e35652f72ea |
| قاعدة البيانات: | OpenAIRE |
Full Text via ClinicalKey | تدمد: | 18760341 |
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