Knockout ofOsSWEET15Impairs Rice Embryo Formation and Seed-Setting

التفاصيل البيبلوغرافية
العنوان: Knockout ofOsSWEET15Impairs Rice Embryo Formation and Seed-Setting
المؤلفون: Zhi Hu, Zhenjia Tang, Jing Yang, Shuhui Bao, Yuanyuan Zhang, Lai Ma, Qingsong Zheng, Fang Yang, Dechun Zhang, Shubin Sun, Yibing Hu
المصدر: Plant and Cell Physiology. 64:258-268
بيانات النشر: Oxford University Press (OUP), 2022.
سنة النشر: 2022
مصطلحات موضوعية: Physiology, Cell Biology, Plant Science, General Medicine
الوصف: We show that the knockout of a sugar transporter gene OsSWEET15 led to a significant drop in rice fertility with around half of the knockout mutant’s spikelets bearing blighted or empty grains. The rest of the spikelets bore fertile grains with a slightly reduced weight. Notably, the ovaries in the blighted grains of the ossweet15 mutants expanded after flowering but terminated their development before the endosperm cellularization stage and subsequently aborted. β- glucuronidase (GUS) and Green Fluorescent Protein (GFP) reporter lines representing the OsSWEET15 expression showed that the gene was expressed in the endosperm tissues surrounding the embryo, which supposedly supplies nutrients to sustain embryo development. These results together with the protein’s demonstrated sucrose transport capacity and plasma membrane localization suggest that OsSWEET15 plays a prominent role during the caryopsis formation stage, probably by releasing sucrose from the endosperm to support embryo development. By contrast, the empty grains were probably caused by the reduced pollen viability of the ossweet15 mutants. Investigation of ossweet11 mutant grains revealed similar phenotypes to those observed in the ossweet15 mutants. These results indicate that both OsSWEET15 and OsSWEET11 play important and similar roles in rice pollen development, caryopsis formation and seed-setting, in addition to their function in seed-filling that was demonstrated previously.
تدمد: 1471-9053
0032-0781
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a9f4578d5576f730c5e78ab1e31b7c41
https://doi.org/10.1093/pcp/pcac173
حقوق: CLOSED
رقم الأكسشن: edsair.doi.dedup.....a9f4578d5576f730c5e78ab1e31b7c41
قاعدة البيانات: OpenAIRE