The branched-chain amino acid aminotransferase TaBCAT1 modulates amino acid metabolism and positively regulates wheat rust susceptibility
| العنوان: | The branched-chain amino acid aminotransferase TaBCAT1 modulates amino acid metabolism and positively regulates wheat rust susceptibility |
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| المؤلفون: | Pilar Corredor-Moreno, Diane G. O. Saunders, Eva Wegel, Andrey V. Korolev, Phoebe E. Davey, Luis A Macías Pérez, Paul Brett, Clare M. Lewis, Lionel Hill, Yvie Morgan, Baldeep Kular, Francesca Minter |
| المساهمون: | School of Medicine [Reno], University of Nevada [Reno], John Innes Ctr, Norwich NR4 7UH, Norfolk, England, Partenaires INRAE, Centre européen de recherche et d'enseignement des géosciences de l'environnement (CEREGE), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Collège de France (CdF (institution))-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), John Innes Centre [Norwich], Biotechnology and Biological Sciences Research Council (BBSRC) |
| المصدر: | Plant Cell The Plant Cell Plant Cell, 2021, 33 (5), pp.1728--1747. ⟨10.1093/plcell/koab049⟩ |
| بيانات النشر: | Oxford University Press (OUP), 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Mutant, Rust (fungus), [SDU.STU]Sciences of the Universe [physics]/Earth Sciences, Plant Science, Stem rust, Models, Biological, 01 natural sciences, Microbiology, 03 medical and health sciences, chemistry.chemical_compound, Gene Expression Regulation, Plant, Cluster Analysis, Homeostasis, Amino Acids, Gene, Pathogen, ComputingMilieux_MISCELLANEOUS, Transaminases, Triticum, Research Articles, Disease Resistance, Plant Diseases, Plant Proteins, 2. Zero hunger, chemistry.chemical_classification, biology, Basidiomycota, Gene Expression Profiling, food and beverages, Cell Biology, Metabolism, biology.organism_classification, Mitochondria, Amino acid, 030104 developmental biology, chemistry, Mutation, Salicylic Acid, Salicylic acid, 010606 plant biology & botany |
| الوصف: | Plant pathogens suppress defense responses to evade recognition and promote successful colonization. Although identifying the genes essential for pathogen ingress has traditionally relied on screening mutant populations, the post-genomic era provides an opportunity to develop novel approaches that accelerate identification. Here, RNA-seq analysis of 68 pathogen-infected bread wheat (Triticum aestivum) varieties, including three (Oakley, Solstice and Santiago) with variable levels of susceptibility, uncovered a branched-chain amino acid aminotransferase (termed TaBCAT1) as a positive regulator of wheat rust susceptibility. We show that TaBCAT1 is required for yellow and stem rust infection and likely functions in branched-chain amino acid (BCAA) metabolism, as TaBCAT1 disruption mutants had elevated BCAA levels. TaBCAT1 mutants also exhibited increased levels of salicylic acid (SA) and enhanced expression of associated defense genes, indicating that BCAA regulation, via TaBCAT1, has a key role in SA-dependent defense activation. We also identified an association between the levels of BCAAs and resistance to yellow rust infection in wheat. These findings provide insight into SA-mediated defense responses in wheat and highlight the role of BCAA metabolism in the defense response. Furthermore, TaBCAT1 could be manipulated to potentially provide resistance to two of the most economically damaging diseases of wheat worldwide. |
| تدمد: | 1532-298X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ab1ac9399e3152ef58bb7bfd2dcad804 https://doi.org/10.1093/plcell/koab049 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....ab1ac9399e3152ef58bb7bfd2dcad804 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1532298X |
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