Involvement of the sigmaN DNA-binding domain in open complex formation
| العنوان: | Involvement of the sigmaN DNA-binding domain in open complex formation |
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| المؤلفون: | Martin Buck, José A. Oguiza, Matthew Chaney, María-Trinidad Gallegos, Wendy Cannon |
| المصدر: | Molecular Microbiology. 33:873-885 |
| بيانات النشر: | Wiley, 1999. |
| سنة النشر: | 1999 |
| مصطلحات موضوعية: | Transcriptional Activation, DNA Footprinting, DNA footprinting, Sigma Factor, Sulfuric Acid Esters, Biology, Microbiology, Bacterial Proteins, Transcription (biology), Enhancer binding, Binding site, Promoter Regions, Genetic, Molecular Biology, RNA polymerase II holoenzyme, Binding Sites, DNA, Superhelical, Single-Strand Specific DNA and RNA Endonucleases, Nucleic Acid Heteroduplexes, DNA-Directed RNA Polymerases, Templates, Genetic, DNA-binding domain, Cell biology, DNA-Binding Proteins, RNA Polymerase Sigma 54, Klebsiella pneumoniae, Biochemistry, Mutation, Transcription preinitiation complex, Sinorhizobium meliloti |
| الوصف: | sigmaN (sigma54) RNA polymerase holoenzyme closed complexes isomerize to open complexes in a reaction requiring nucleoside triphosphate hydrolysis by enhancer binding activator proteins. Here, we characterize Klebsiella pneumoniae sigmaN mutants, altered in the carboxy DNA-binding domain (F354A/F355A, F402A, F403A and F402A/F403A), that fail in activator-dependent transcription. The mutant holoenzymes have altered activator-dependent interactions with promoter sequences that normally become melted. Activator-dependent stable complexes accumulated slowly in vitro (F402A) and to a reduced final level (F403A, F402A/F403A, F354A/F355A). Similar results were obtained in an assay of activator-independent stable complex formation. Premelted templates did not rescue the mutants for stable preinitiation complex formation but did for deleted region I sigmaN, suggesting different defects. The DNA-binding domain substitutions are within sigmaN sequences previously shown to be buried upon formation of the wild-type holoenzyme or closed complex, suggesting that, in the mutants, alteration of the sigmaN-core and sigmaN-DNA interfaces has occurred to change holoenzyme activity. Core-binding assays with the mutant sigmas support this view. Interestingly, an internal deletion form of sigmaN lacking the major core binding determinant was able to assemble into holoenzyme and, although unable to support activator-dependent transcription, formed a stable activator-independent holoenzyme promoter complex on premelted DNA templates. |
| تدمد: | 1365-2958 0950-382X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ab70745b1dbdcf0be409a530350ed1ad https://doi.org/10.1046/j.1365-2958.1999.01542.x |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....ab70745b1dbdcf0be409a530350ed1ad |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13652958 0950382X |
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