Changes in brain tissue oxygenation after treatment of diffuse traumatic brain injury by erythropoietin
| العنوان: | Changes in brain tissue oxygenation after treatment of diffuse traumatic brain injury by erythropoietin |
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| المؤلفون: | Anne Millet, Lucie Gaide-Chevronnay, Pierre Bouzat, Emmanuel L. Barbier, Thibaut Trouve-Buisson, Karin Pernet-Gallay, Yvonnick Boué, Jean François Payen |
| المساهمون: | INSERM U836, équipe 5, Neuroimagerie fonctionnelle et perfusion cérébrale, Grenoble Institut des Neurosciences (GIN), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Pôle Anesthésie Réanimation, CHU Grenoble-Hôpital Michallon-CHU Grenoble-Hôpital Michallon, Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Département de Réanimation Pédiatrique, Hôpital Couple-Enfant-Hôpital Couple-Enfant, Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), Pôle Anesthésie Réanimation, CHU Grenoble-Hôpital Michallon, Neuro-imagerie fonctionnelle et métabolique (ANTE-INSERM U836, équipe 5), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), INSERM, CNRS, Fondation 'Gueules cassées', Association pour la Recherche en Néonatologie, Ministère de l'Education, de la Recherche et de la Technologie, Dojat, Michel |
| المصدر: | Critical Care Medicine Critical Care Medicine, Lippincott, Williams & Wilkins, 2013, 41 (5), pp.1316-24. ⟨10.1097/CCM.0b013e31827ca64e⟩ |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Male, MESH: Brain Edema, MESH: Random Allocation, [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, Perfusion scanning, Brain Edema, Critical Care and Intensive Care Medicine, 030218 nuclear medicine & medical imaging, Brain Ischemia, Brain ischemia, Random Allocation, 0302 clinical medicine, MESH: Risk Factors, Reference Values, Risk Factors, Edema, MESH: Microcirculation, MESH: Animals, MESH: Oxygen Consumption, Infusions, Intravenous, Neocortex, MESH: Reference Values, MESH: Brain Ischemia, Brain, MESH: Cerebrovascular Circulation, 3. Good health, medicine.anatomical_structure, Cerebral blood flow, Anesthesia, Cerebrovascular Circulation, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], medicine.symptom, medicine.drug, MESH: Rats, Traumatic brain injury, MESH: Diffusion Magnetic Resonance Imaging, MESH: Brain, 03 medical and health sciences, Oxygen Consumption, medicine, Animals, Humans, [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], MESH: Erythropoietin, Rats, Wistar, MESH: Infusions, Intravenous, Erythropoietin, MESH: Humans, business.industry, Microcirculation, [SDV.NEU.NB] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, MESH: Rats, Wistar, Oxygenation, medicine.disease, MESH: Male, Rats, Disease Models, Animal, Diffusion Magnetic Resonance Imaging, Brain Injuries, MESH: Brain Injuries, MESH: Disease Models, Animal, business, 030217 neurology & neurosurgery |
| الوصف: | International audience; OBJECTIVES: To investigate the effects of recombinant human erythropoietin on brain oxygenation in a model of diffuse traumatic brain injury. DESIGN: Adult male Wistar rats. SETTING: Neurosciences and physiology laboratories. INTERVENTIONS: Thirty minutes after diffuse traumatic brain injury (impact-acceleration model), rats were intravenously administered with either a saline solution or a recombinant human erythropoietin (5000 IU/kg). A third group received no traumatic brain injury insult (sham-operated). MEASUREMENTS AND MAIN RESULTS: Three series of experiments were conducted 2 hours after traumatic brain injury to investigate: 1) the effect of recombinant human erythropoietin on brain edema using diffusion-weighted magnetic resonance imaging and measurements of apparent diffusion coefficient (n = 11 rats per group); local brain oxygen saturation, mean transit time, and blood volume fraction were subsequently measured using a multiparametric magnetic resonance-based approach to estimate brain oxygenation and brain perfusion in the neocortex and caudoputamen; 2) the effect of recombinant human erythropoietin on brain tissue PO₂ in similar experiments (n = 5 rats per group); and 3) the cortical ultrastructural changes after treatment (n = 1 rat per group). Compared with the sham-operated group, traumatic brain injury saline rats showed a significant decrease in local brain oxygen saturation and in brain tissue PO₂ alongside brain edema formation and microvascular lumen collapse at H2. Treatment with recombinant human erythropoietin reversed all of these traumatic brain injury-induced changes. Brain perfusion (mean transit time and blood volume fraction) was comparable between the three groups of animals. CONCLUSION: Our findings indicate that brain hypoxia can be related to microcirculatory derangements and cell edema without evidence of brain ischemia. These changes were reversed with post-traumatic administration of recombinant human erythropoietin, thus offering new perspectives in the use of this drug in brain injury. |
| وصف الملف: | application/pdf |
| تدمد: | 1530-0293 0090-3493 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ab7842f6a0ac39468c62d7d550eb53bd https://pubmed.ncbi.nlm.nih.gov/23591224 |
| حقوق: | EMBARGO |
| رقم الأكسشن: | edsair.doi.dedup.....ab7842f6a0ac39468c62d7d550eb53bd |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15300293 00903493 |
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