Molecular mechanism of cargo recognition and handover by the mammalian signal recognition particle
| العنوان: | Molecular mechanism of cargo recognition and handover by the mammalian signal recognition particle |
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| المؤلفون: | Kan Kobayashi, Zikun Zhu, Shu-ou Shan, Sowmya Chandrasekar, Simon Eitzinger, Nenad Ban, Ahmad Jomaa |
| المصدر: | Cell reports Cell Reports, Vol 36, Iss 2, Pp 109350-(2021) Cell Reports, 36 (2) |
| بيانات النشر: | ETH Zurich, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Signal peptide, Models, Molecular, Receptors, Peptide, QH301-705.5, co-translational protein targeting, cryoelectron microscopy, Receptors, Cytoplasmic and Nuclear, GTPase, medicine.disease_cause, Ribosome, Models, Biological, environment and public health, General Biochemistry, Genetics and Molecular Biology, Article, GTP Phosphohydrolases, Protein Domains, Protein targeting, medicine, Animals, Humans, signal recognition particle, signal peptide, Biology (General), Ribosome-nascent chain complex, Mammals, Signal recognition particle, GTPases, Bacteria, Chemistry, Endoplasmic reticulum, RNA, transmembrane domain, ribosome nascent chain complex, endoplasmic reticulum, Cell biology, Protein Transport |
| الوصف: | Co-translational protein targeting to membranes by the signal recognition particle (SRP) is a universally conserved pathway from bacteria to humans. In mammals, SRP and its receptor (SR) have many additional RNA features and protein components compared to the bacterial system, which were recently shown to play regulatory roles. Due to its complexity, the mammalian SRP targeting process is mechanistically not well understood. In particular, it is not clear how SRP recognizes translating ribosomes with exposed signal sequences and how the GTPase activity of SRP and SR is regulated. Here, we present electron cryo-microscopy structures of SRP and SRP·SR in complex with the translating ribosome. The structures reveal the specific molecular interactions between SRP and the emerging signal sequence and the elements that regulate GTPase activity of SRP·SR. Our results suggest the molecular mechanism of how eukaryote-specific elements regulate the early and late stages of SRP-dependent protein targeting. Cell Reports, 36 (2) ISSN:2666-3864 ISSN:2211-1247 |
| وصف الملف: | application/pdf; application/application/pdf |
| اللغة: | English |
| تدمد: | 2666-3864 2211-1247 |
| DOI: | 10.3929/ethz-b-000495624 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::abe7f786268d341e65cdfb60a550641f |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....abe7f786268d341e65cdfb60a550641f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 26663864 22111247 |
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| DOI: | 10.3929/ethz-b-000495624 |