ALW peptide ameliorates lupus nephritis in MRL/lpr mice
العنوان: | ALW peptide ameliorates lupus nephritis in MRL/lpr mice |
---|---|
المؤلفون: | Lingling Peng, Mei Lu, Kunyi Wu, Huixia Wang, Yumin Xia, Siyue Zhai, Jie Yang |
المصدر: | Arthritis Research & Therapy Arthritis Research & Therapy, Vol 21, Iss 1, Pp 1-14 (2019) |
بيانات النشر: | Springer Science and Business Media LLC, 2019. |
سنة النشر: | 2019 |
مصطلحات موضوعية: | 0301 basic medicine, Mice, Inbred MRL lpr, medicine.medical_specialty, lcsh:Diseases of the musculoskeletal system, Anti-dsDNA IgG, Lupus nephritis, Inflammation, urologic and male genital diseases, Kidney, Immunoglobulin G, Pathogenesis, Mice, 03 medical and health sciences, 0302 clinical medicine, immune system diseases, Fibrosis, Internal medicine, medicine, Animals, skin and connective tissue diseases, 030203 arthritis & rheumatology, biology, ALW peptide, Chemistry, Molecular Mimicry, medicine.disease, Lupus Nephritis, 030104 developmental biology, medicine.anatomical_structure, Endocrinology, Antibodies, Antinuclear, biology.protein, Mesangial proliferative glomerulonephritis, Female, lcsh:RC925-935, Antibody, medicine.symptom, Peptides, Research Article |
الوصف: | Background Lupus nephritis (LN) is a common and serious complication of systemic lupus erythematosus. Anti-double-stranded (ds) DNA immunoglobulin G (IgG) plays a pivotal role in the pathogenesis of LN. Currently, there are various therapies for patients with LN; however, most of them are associated with considerable side effects. We confirmed previously that ALW (ALWPPNLHAWVP), a 12-amino acid peptide, inhibited the binding of polyclonal anti-dsDNA antibodies to mesangial cells and isolated glomeruli in vitro. In this study, we further investigate whether the administration of ALW peptide decreases renal IgG deposition and relevant damage in MRL/lpr lupus-prone mice. Methods Forty female MRL/lpr mice were randomly divided into four groups. The mice were intravenously injected with D-form ALW peptide (ALW group), scrambled peptide (PLP group), and normal saline (NaCl group) or were not treated (blank group). The IgG deposition, the histopathologic changes, and the expressions of profibrotic factors were analyzed in the kidney of MRL/lpr mice. Results Compared with the other groups, glomerular deposition of IgG, IgG2a, IgG2b, and IgG3 was decreased in the ALW group. Moreover, ALW administration attenuated renal histopathologic changes in MRL/lpr mice, including mesangial proliferation and infiltration of inflammatory cells. Furthermore, the expressions of profibrotic cytokines, such as transforming growth factor-beta1 (TGF-β1) and platelet-derived growth factor B (PDGF-B), decreased in the serum and kidney tissue of ALW-treated mice. Conclusions Our study demonstrated that ALW peptide ameliorates the murine model of LN, possibly through inhibiting renal IgG deposition and relevant tissue inflammation and fibrosis. |
تدمد: | 1478-6362 |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ac5d0f9d4c246597afefdc6273099059 https://doi.org/10.1186/s13075-019-2038-0 |
حقوق: | OPEN |
رقم الأكسشن: | edsair.doi.dedup.....ac5d0f9d4c246597afefdc6273099059 |
قاعدة البيانات: | OpenAIRE |
تدمد: | 14786362 |
---|