Deficiency of GD3 Synthase in Mice Resulting in the Attenuation of Bone Loss with Aging
| العنوان: | Deficiency of GD3 Synthase in Mice Resulting in the Attenuation of Bone Loss with Aging |
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| المؤلفون: | Koichi Furukawa, Shigemi Goto, Yoshitaka Mishima, Takuma Sato, Ken Miyazawa, Kenjiro Tanaka, Akifumi Togari, Kazunori Hamamura, Kosuke Hamajima, Hironori Mori, Hisataka Kondo, Shoyoku Yo |
| المصدر: | International Journal of Molecular Sciences Volume 20 Issue 11 International Journal of Molecular Sciences, Vol 20, Iss 11, p 2825 (2019) |
| بيانات النشر: | MDPI AG, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Aging, medicine.medical_specialty, glycosylation, Osteoclasts, Article, Catalysis, Bone resorption, Bone remodeling, lcsh:Chemistry, Inorganic Chemistry, Mice, Osteogenesis, Osteoclast, Internal medicine, medicine, Animals, Bone Resorption, Physical and Theoretical Chemistry, Receptor, lcsh:QH301-705.5, Molecular Biology, Cells, Cultured, Spectroscopy, Ganglioside, glycosphingolipids, biology, Chemistry, RANK Ligand, Organic Chemistry, Osteoblast, General Medicine, Sialyltransferases, gangliosides, Computer Science Applications, RAW 264.7 Cells, medicine.anatomical_structure, Endocrinology, lcsh:Biology (General), lcsh:QD1-999, RANKL, biology.protein, bone metabolism, Cancellous bone |
| الوصف: | Gangliosides are widely expressed in almost all tissues and cells and are also considered to be essential in the development and maintenance of various organs and tissues. However, little is known about their roles in bone metabolism. In this study, we investigated the effects of genetic deletion of ganglioside D3 (GD3) synthase, which is responsible for the generation of all b-series gangliosides, on bone metabolism. Although b-series gangliosides were not expressed in osteoblasts, these gangliosides were expressed in pre-osteoclasts. However, the expression of these gangliosides was decreased after induction of osteoclastogenesis by receptor activator of nuclear factor kappa-B ligand (RANKL). Three-dimensional micro-computed tomography (3D-&mu CT) analysis revealed that femoral cancellous bone mass in GD3 synthase-knockout (GD3S KO) mice was higher than that in wild type (WT) mice at the age of 40 weeks, although there were no differences in that between GD3S KO and WT mice at 15 weeks old. Whereas bone formation parameters (osteoblast numbers/bone surface and osteoblast surface/bone surface) in GD3S KO mice did not differ from WT mice, bone resorption parameters (osteoclast numbers/bone surface and osteoclast surface/bone surface) in GD3S KO mice became significantly lower than those in WT mice at 40 weeks of age. Collectively, this study demonstrates that deletion of GD3 synthase attenuates bone loss that emerges with aging. |
| وصف الملف: | application/pdf |
| تدمد: | 1422-0067 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ad2a06dc6d09dbb79c238bd448e8db21 https://doi.org/10.3390/ijms20112825 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....ad2a06dc6d09dbb79c238bd448e8db21 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14220067 |
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