Liquid Chromatographic Determination of Ornithine and Lysine Based on Intramolecular Excimer-forming Fluorescence Derivatization
| العنوان: | Liquid Chromatographic Determination of Ornithine and Lysine Based on Intramolecular Excimer-forming Fluorescence Derivatization |
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| المؤلفون: | Hideyuki Yoshida, Hitoshi Nohta, Yukitaka Nakano, Masatoshi Yamaguchi, Junichi Ishida, Katsumi Koiso |
| المصدر: | Analytical Sciences. 17:107-112 |
| بيانات النشر: | Springer Science and Business Media LLC, 2001. |
| سنة النشر: | 2001 |
| مصطلحات موضوعية: | Ornithine, Detection limit, Pyrenes, Chromatography, Lysine, Reproducibility of Results, Excimer, Fluorescence, Mass Spectrometry, Fluorescence spectroscopy, Analytical Chemistry, chemistry.chemical_compound, Spectrometry, Fluorescence, chemistry, Reagent, Calibration, Humans, Pyrene, Indicators and Reagents, Derivatization, Chromatography, Liquid, Fluorescent Dyes |
| الوصف: | A highly sensitive and selective fluorometric determination method for ornithine and lysine has been developed. This method is based on an intramolecular excimer-forming fluorescence derivatization with a pyrene reagent, 4-(1-pyrene)butyric acid N-hydroxysuccinimide ester (PSE), followed by reversed-phase liquid chromatography (LC). The analytes, containing two amino moieties in a molecule, were converted to the corresponding dipyrene-labeled derivatives by reaction with PSE. The derivatives afforded intramolecular excimer fluorescence (450-550 nm) which can clearly be discriminated from the normal fluorescence (370-420 nm) emitted from PSE and monopyrene-labeled derivatives of monoamines. The structures of the derivatives and the emission of excimer fluorescence were confirmed by LC with mass spectrometry and with three-dimensional fluorescence detection system, respectively. The PSE derivatives of ornithine and lysine could be separated by reversed-phase LC on ODS column with isocratic elution. The detection limits (signal-to-noise ratio = 3) for ornithine and lysine were 3.5 and 3.7 fmol, respectively, for a 20-microl injection. Furthermore, this method had enough selectivity and sensitivity for the determination of ornithine and lysine in normal human urine. |
| تدمد: | 1348-2246 0910-6340 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ad32857c2cfb0a1d503039638eae40a9 https://doi.org/10.2116/analsci.17.107 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....ad32857c2cfb0a1d503039638eae40a9 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 13482246 09106340 |
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